Figure 2. Acylated ghrelin concentrations and hunger over 240 min during the exercise and control trials. There was an effect of trial (p = 0.032), an effect of time (p < 0.05), and a trial x time interaction (p = 0.005) for acylated ghrelin. Subject hunger ratings (mean ± SEM) response to the question "How hungry do you feel right now?" on a VAS from 0 to 100 (0 = not hungry at all; 100 = extremely hungry). Two-factor ANOVA revealed a main effect of trial (P = 0.013), a main effect of time (p < 0.05), and a trial and time interac-tion (p = 0.005) for hunger *Significantly different from the control trial (p < 0.05) after Bonferroni adjustment.