KEY WORDS: Mood, coping, environment, altitude, cold, performance.

Participants
Eight male elite cyclists (Age: M = 26.23 yrs., SD = 6.74) completed the hypoxia-normal cycling trials. Participants reported to train for a rounded average of 14.45 hours per week (SD = 1.54). Ten male highly trained cyclists (Age: M = 23.34, SD = 5.45 years, range 18-35 years) participated in the cold-normal trials. Participants trained for a rounded average of 14 hours per week (SD = .23 hours). All participants had competed internationally and reported to cycle regularly in the UK outside during the year, and therefore experience training in cold conditions in the winter months. All athletes reported to have had some experience of training at altitude, but none had been at altitude in the previous year.

Measures
Mood was assessed using the Brunel Mood Scale (BRUMS: Terry et al., 1999; 2003). The BRUMS assesses anger, confusion, depression, fatigue, tension, and vigor, hence is a derivative of the Profile of Mood States (McNair et al., 1971; 1992). Anger items include "Bad-tempered" and "Angry", Confusion items include "Muddled" and "Uncertain", Depression items include "Depressed" and "Miserable", Fatigue items include "Sleepy" and "Tired", Tension items include "Anxious" and "Panicky", and Vigor items include "Lively" and "Energetic". Items are rated on a 5-point scale anchored by "not at all" (0) and "extremely" (4). Validation of the BRUMS involved 3,361 participants ranging in age from 12-39 years (Terry et al., 1999; 2003). Confirmatory factor analysis supported the factorial validity of a 24-item six-factor model using both independent and multisample analyses among athletic samples. Lane and Terry (1998) found the BRUMS scores predicted time trial cycling performance.

Cycling performance test
Participants performed two-hour (approximately 50-mile) cycle bouts on a stationary cycle ergometer rig (Kingcycle, High Wycombe, UK) at an equivalent power output to lactate threshold (previously determined in normal laboratory conditions at 19°C). The normal condition involved cycling in a laboratory at 19°C. The hypoxic trial was completed in a commercially available hypoxic chamber (Edge 4, London, UK). The chamber reduces the F_IO₂ via a ventilation system that simultaneously draws both ambient air (≈20.93% O₂ and ≈79.0% N₂) and a gas comprised of 100% N₂ into the chamber. This leads to an intra-chamber gas composition of ≈15.3% O₂ and ≈84.7% N₂, approximately equivalent to an altitude of 2500m. The 0°C trial was completed in a commercially available climatic chamber (SANYO, Gallenkamp PLC, Loughborough, U.K).

Procedure
The institution of the first author granted ethical approval. Participants gave informed written consent before testing. The BRUMS was completed immediately before cycling, after the first hour, and again after the second hour before stopping. Participants completed the BRUMS using the "How do you feel right now?" instructional set. Participants performed the test individually with only the experimenters in the laboratory hence, the potentially motivating effects of other riders was controlled. Participants were freely allowed to drink water during all trials. Both cycle trials were separated by two weeks.

Data were analyzed by comparing mood state changes over time (pre, 1 hour, 2 hours, and post) by environment condition (cold vs hypoxia vs normal) using multivariate analysis of variance. A preliminary data analysis check was made on mood changes between the two normal conditions.

Initial physiological testing indicated that participants were well-trained athletes with an average VO_2max score of 65.6 (SD = 7 ml·kg^-1·min^-1). The wattage associated with lactate threshold (M = 226.7, SD = 28.3 watts) indicated that participants could sustain a relatively high level of workload from aerobic sources.

MANOVA results indicated no significant interaction effect for mood changes over time by environment condition (Wilks' Lambda = .72, p = 0.14, Eta² = 0.08, a significant main effect for mood changes over time (Table 1, Wilks' Lambda = 0.57, p < 0.001, Eta² = 0.25) and a significant main effect for differences in mood by condition (Wilks' Lambda = 0.65, p < 0.001, Eta² = 0.19). Although the multivariate effect was not significant, univariate interaction effects were found for anger (F = 3.70, p < 0.01, Eta² = .13) and fatigue (F = 2.75, p < 0.05, Eta² = 0.10) where anger and fatigue increased significantly more during exercise at altitude after the first hour.

Univariate results were evident for mood changes over time for anger (F = 4.15, p < 0.05, Eta² = 0.08) and fatigue over time (F = 16.63, p < 0.001, Eta² = 0.26) with significant increases in fatigue following the second hour of exercise. Univariate results for the effects of environment on mood indicated anger (F = 13.41, p < 0.001, Eta² = 22), confusion (F = 6.04, p < 0.01, Eta² = 0.11) depression (F = 7.53, p < 0.01, Eta² = .14), fatigue (F = 11.84, p < 0.001, Eta² = .20) and tension (F = 6.95, p < 0.01, Eta² = 0.13) scores were significantly higher at altitude than either cold or normal conditions.

The present study examined changes in mood states during a two-hour cycle performance in adverse environmental conditions. Results demonstrated that performing intense exercise was associated with increased fatigue regardless of environment conditions. Results for changes in anger over time indicated that it increased significantly after an hour of exercise when performing at altitude, with no significant differences in anger in cold and normal conditions.

Results also showed that an equivalent altitude of 2500m was associated with increased anger, confusion, depression, fatigue, and tension. Findings of the present study lend some support for previous research that shows increased negative mood with hypoxia (Elmore and Evans, 1983; Banderet and Burse, 1991; Bahrke and Shukitt-Hale, 1993; Bonnon et al., 1999; Piehl-Aulin et al., 1998; Bolmont et al., 2000). Lane et al. (2004) argued that stress-responses to extreme environments become exacerbated among athletes used to performing at sea level who are required to perform at extreme conditions on an irregular basis. Lane et al. (2003) used this argument in their work with biathletes in preparation for the 2002 Winter Olympic Games. Findings of the present study lend support to the benefits of acclimatising to altitude for reducing the likelihood of athletes experiencing negative mood during intense exercise.

Results indicated that there was no significant difference in mood states between performing in the cold and normal conditions. Although previous research has found evidence to suggest that negative mood states are associated with performing strenuous exercise in cold environments (Kobrick and Johnson, 1991), this has not been consistently reported. Indeed, Acevedo and Ekkekakis (2001) argued that performing intense exercise in the cold might be beneficial to some athletes as it serves as a heat reduction strategy. It should be noted that athletes in the present study where able to drink water ad libatum, and therefore this might have lead to discrepancies in sweat production and heat dissipation. It is possible that ad-lib drinking in the present study could counter-balance the proposed motivational effects of performing in the cold. Future research should control fluid ingestion across trials.

It is possible that non-significant effects for cold and significant effects for altitude could be explained by the previous experiences of athletes within the samples. Participants were experienced cyclists who trained regularly in the UK. Typical changes in the weather in the UK would mean that if participants ride on a regular basis, they should be familiar with riding in cold conditions similar to those used in the present study. Recent research indicates that teaching athletes how to cope with altitude by training in an altitude chamber whilst living under normal conditions is associated with enhanced mood states in hypoxic conditions (Lane et al., 2003; Whyte et al., 2002). It is suggested that athletes can learn strategies to regulate mood states associated with altitude, something that athletes in the present study were less likely to have done. It is suggested that similar mood responses could have occurred if athletes were equally familiar with performing at altitude as they were in the cold.

Acevedo and Ekkekakis (2001) argued that research should investigate affective changes in response to exercise in extreme conditions using a transactional design in which the mood states responses at one point in time are important sources of information in how athletes cope with situational and environmental stressors. Studies that test transactional research designs are rare due the complexity and difficulty of taking multiple measures at different time points during performance. However, mood researchers have emphasized the transitory nature of mood states, and the importance of investigating how mood states interact (Lane and Terry, 2000). The present study showed that anger and fatigue increased over time. Findings for fatigue should be not be surprising given athletes reported low scores at the start of the investigation, and it is reasonable to expect fatigue to increase in response to strenuous exercise. It is suggested that athletes used anger as a strategy to raise energy levels when performing at altitude. Lane and Terry (2000) argued that some individuals could learn to regulate anger to optimum levels, and use anger to enhance determination.

We suggest that there is need for future research to investigate mood state changes to strenuous exercise. We argue that future research is needed to address the acknowledged limitations within the present study. Acevedo and Ekkekakis (2001) suggested investigated affective changes using psycho-physiological model. Although research has tested each part of the model separately, research has not tested the entire model simultaneously. It is suggested that future research tests the psycho-physiological model proposed by Acevedo and Ekkekakis (2001). A second line of future research could test the effectiveness of mood-regulating strategies on mood state responses to exercise in extreme conditions. There is a need for well-controlled studies to explore this proposal, however, we suggest that future research utilizes an ecologically valid design. A limitation of the present study is that athletes performed in laboratory conditions, and although this allows for the control of potentially confounding variables such as changes in environment conditions during an event, the mindset linked with a laboratory trial is likely to differ than before an important competition.

Collectively, it is suggested that future research attempts to explore the mechanisms through which athletes learn to cope with performing in extreme conditions.

• The present study found that mood state changes were more pronounced when performing at a simulated altitude of 2,500 metres than performing in the cold and normal laboratory conditions at a pace equivalent of lactate threshold.
• Findings from the present study indicate that that altitude is associated with negative mood states,
• Results show that mood states change during extreme exercise with increases most notably in fatigue and reductions in vigor. It should be noted that environment conditions did not affect the change in mood states over time.
• We suggest that further research is needed to explore mechanisms that individuals use to regulate negative mood during strenuous exercise.