KEY WORDS: Energy, intake, expenditure, carbohydrate, fluid, micronutrient.

Good nutritional practice is essential to athletic success by improving the quality of training, maximising performance and speeding recovery time. Soccer is described as a high intensity intermittent sport involving continual changes in activity (Hargreaves, 1994). Elite female players have been reported to cover total distances of 8.5km, 9.5km (Bangsbo, 1994) and 12.4km (Holmes, 2002) with a maintained relative exercise intensity of 70% VO₂max (Brewer, 1994) despite spending 76% and 11% of their time in low and high intensity activities respectively (Holmes, 2002). Such high metabolic and energy demands of soccer training and
competition must be met by adequate nutritional intake. Previous research of the nutritional practices of female soccer players is limited but have reported daily energy intakes ranging from 1778 ± 715 to 2290 ± 310 kcal·day^-1 (Clark et al., 2003; Scott et al., 2003) with percentage carbohydrate, protein and fat intakes of 47.8 ± 9.8 to 55.0 ± 7.5%, 13.9 ± 4.0 to 15.0 ± 3.1 and 29.0 ± 5.7 to 33.3 ± 9.5 respectively (Scott et al., 2003; Clark et al., 2003). Furthermore, the nutritional practice of female athletes should be concerned with gender specific considerations of iron and calcium (Piehl-Aulin, 1995). Clark et al., 2003 reported that NCAA Division 1 players failed to meet the Daily Recommended Intakes for both of these essential minerals, whilst Scott et al., 2003also reported iron deficiencies.


The validity of data collected via self- reported food diaries have been questioned due to inherent limitations in the technique. These include frequent bias towards underestimation of habitual intake (Black et al., 1991), failure to report all foods consumed (Goldberg et al., 1991) and/or modification of the normal eating pattern (Stockley, 1985). In addition to this, Bingham, 1987 stated that seven days is the shortest time necessary to cover fluctuations in dietary patterns e.g. weekdays and weekends, and the longest time participants can be expected to co-operate. Indeed, both of the aforementioned studies may be limited in that they have collected data over relatively short time frames; 2 days not including weekends (Scott et al., 2003) and 3 days incorporating weekends (Clark et al., 2003). Previous research is further limited by failing to provide parallel activity data to indicate energy expenditure, which would enhance the interpretation of nutritional intake. The current study attempts to address some of these limitations by recording nutritional intakes for 7 days as well as reporting the corresponding activity patterns during this timeframe.

Thus the aim of the present study was to further investigate the nutritional practices of elite female soccer players by reporting diet and activity records over a period of seven days.

Participants
Sixteen international female soccer players (Age = 25.5 ± 3.9 yrs; Height = 1.67 ± 0.08 m; Body Mass = 61.5 ± 5.3 kg) participated in the investigation. Players were fully informed of the purpose and procedures of the investigation and provided consent at the outset.

Dietary and activity collection
The participants' dietary intake and activity levels were collected, via self-reporting diaries, during the second half of the 2003/04 competitive season when all players were participating in full training and competition. Participants were required to record all food and beverage intake, and any training, matches or other physical activities for a period of seven-days. Reporting over seven-days provides the best opportunity to collect valid information as it encompasses the potential diversity of diet and activity practices of weekdays and weekends (Bingham, 1987; Tilgner and Schiller, 1991).

Players attended a training camp immediately prior to the collection period. During this time they were issued with a food and activity diary along with verbal and written instructions for its completion. Information provided focused on average portion sizes for a range of common foods (e.g. pasta, rice, cereals) and a guide to universal household measures (e.g. teaspoon, tablespoon, cup) to improve the estimation of daily intake.

The food section of the diary included the following headings; 'meal', 'food/beverage description', 'portion size/quantity', 'brand', 'food type' and 'cooking method' to facilitate accurate analysis. The activity section requested that participants record the type, duration, and approximate intensity of each activity on a daily basis. Participants were requested to follow their customary eating patterns during the prospective recording days and were asked to confirm this via a series of brief questions at the end of the food and activity diary. Finally space was provided for participants to include any other information including details of any dietary supplements consumed.

On completion of the seven-day recording period, participants were requested to return their completed diary for analysis. Dietary analysis was undertaken by a single researcher using the computerised package 'Dietmaster 4. 0' (Stuart Dyson Associates, London, UK). Estimation of energy expenditure was undertaken by a second single researcher by calculating BMR (COMA, 1991) alongside analysis of reported activities using the computerised package 'Diet Organizer 2. 0' (Mulberrysoft, USA). The use of a single researcher for these analyses enhanced reliability of data by minimising potential variations in the interpretation of food or activity reports (Deakin, 2000).

Data analysis
A seven-day average for total energy intake (kcals), total energy expenditure (kcals), macronutrients (g·kg^-1, %), micronutrients (mg, ug) and fluid intake (L) was determined for each participant. Descriptive statistics were determined for all variables. Paired samples t-tests were used to assess energy balance (energy intake vs. energy expenditure), and to identify possible nutrient deficiencies via comparison to Reference Nutrient Intakes (COMA, 1991).

Energy balance
The average daily intake from the 7-day analysis was 1904 ± 366 kcal, and the mean energy expenditure was 2154 ± 596 kcal. This represents a 250 kcal energy deficit, though statistical analysis reveals no significant difference between intake and expenditure (p > 0.05), suggesting average energy balance was achieved.

The relative energy intake of 30.9 ± 5.5 kcal·kg^-1·day^-1 falls below the recommendations for female soccer players of 47-60 kcal.kg^-1.day^-1 (Economos et al., 1993). This reported intake was significantly lower (p < 0.05) than the lower end of this recommended range (47 kcal·kg^-1·day^-1). Average daily training time was 108.2 ± 35.9 minutes, which represents 31.8 ± 6.5% of the total daily energy expenditure. There was no significant difference (p > 0.05) in body mass recorded before and after the 7-day report period (61.5 ± 5.3kg vs. 61.6 ± 5.3kg).

Macro-nutrients
The mean intakes of the energy producing macronutrients were 53.8 ± 6.8%, 28.8 ± 6.6% and 16.8 ± 2.1% for carbohydrate, fat and protein, respectively. When reported as a percentage of total calories, carbohydrate and protein intakes were significantly lower and higher (p < 0.05) respectively of the recommended levels. Fat intake was significantly higher (p < 0.05) than the lower value in the recommended range (18%), but not significantly different (p > 0.05) to the upper end of this range (28%). When macro-nutrient intake is expressed as g·kg^-1·day^-1 (Table 1), carbohydrate and protein intakes fell within their recommended ranges.

Micro-nutrients
With the exception of Vitamin A (56% of RNI) and iron (82% of RNI), all micronutrient intakes are above the RNI. Calcium and iron intakes are not significantly different (p > 0.05; Table 2) from the RNI.

Mean fluid intake was 2466 ± 1350 ml·day^-1, which represents 99% of the baseline daily requirement proposed by Maughan, 2000.

Energy
The results of this nutritional analysis of elite women's soccer players demonstrate an average daily intake, recorded over 7 days, to be 1904 ± 366 kcal. This is lower than previously reported values for female soccer players (Clark et al., 2003), and when expressed relative to body weight (30.9 ± 5.5 kcal·kg^-1·day^-1) falls significantly below (p < 0.05) previous recommendations for female soccer players (47-60 kcal·kg^-1·day^-1, Economos et al., 1993).

Energy expenditure data (2154 ± 596 kcal·day^-1) present a 250 kcal·day^-1 energy deficit to intake (1904 ± 366 kcal·day^-1). Statistical analysis revealed no significant difference between intake and expenditure (p > 0.05), suggesting average energy balance was achieved. The current findings are similar to those previously reported. Fogelholm et al. (1994) reported daily energy intake of 2131 ± 400 kcal with a 111 ± 450 kcals energy deficit in normal weight female soccer players. The achievement of energy balance is further supported by there being no significant change in body mass pre and post the reporting period (61.5 ± 5.3kg vs. 61.6 ± 5. 3kg, p > 0.05), however it is recognised that this observation exists only within the limitations of the assessment techniques used. However, the non-significant calorie deficit of 248 kcals may have a number of detrimental practical implications. Regular energy deficit over time may lead to a decrease in body mass, including the potential loss of muscle mass, which would be detrimental to performance. The energy deficit reported in this study (248 kcal·day^-1) would result in very gradual weight loss of 0.03kg·week^-1 (McArdle et al., 1999), which is too small to be observed within the sensitivity of standard weighing scales but would be significant over time. Furthermore, a negative energy balance can also impact upon recovery rates, training adaptations, cognitive functioning and the immune system (O'Connor et al., 2000) that would all be detrimental to a players capability to undertake training and competition performance especially at an international level where demands are higher.

Macro-nutrients
Carbohydrate is the primary fuel substrate during soccer, and consequently high dietary intakes of 60-70% of total calorific intake have been recommended for athletes (Devlin and Williams, 1995) and footballers (Schokman et al., 1999). In the present study carbohydrate intake was significantly lower than these recommendations (53.8 ± 6.8%, p < 0.05), but fall within the range previously reported for female soccer players 47.8 ± 9.8 to 55.0 ± 7.5% (Clark et al., 2003; Scott et al., 2003). When expressed relative to body mass the daily carbohydrate intake of 4.0 g·kg^-1·day^-1 just meet the recommended 4.0 - 6.0 g·kg^-1·day^-1 (Williams, 1995). The current data provide further evidence to the commonly observed low carbohydrate intakes that are insufficient for adequate glycogen re-synthesis in female athletes (Nutter, 1991; Tanaka et al., 1995). Dietary recommendations for promoting maximal glycogen resynthesis and for the maintenance of muscle glycogen levels are to consume a high carbohydrate diet, 55 - 65% (Clark, 1994) and a daily intake of 7 - 10 g·kg^-1·day^-1 (Schokman et al., 1999). Based upon these recommendations players in the current study reported carbohydrate intakes that are likely to be inadequate to replace muscle and liver glycogen stores which rapidly deplete during repeated bouts of high intensity exercise performed during training and competition (Bangsbo, 1994). Ultimately both the quality of training and match performance may be compromised on a regular basis due to earlier onset of fatigue.

To alleviate this, it appears that players in the current study need to consider increasing their daily carbohydrate intake. However, Clark et al., 2003 identified that the typically low daily calorific intake of female athletes (<2000 kcals·day^-1) are insufficient to follow the recommended guidelines of ~60% carbohydrate, as this will only provide 4.0 - 5.0 g·kg^-1·day^-1. Similarly, Economos et al., 1993 recommended a minimum of 6.0 g·kg^-1·day^-1 for athletes consuming less than 45 kcal·kg^-1.day^-1, which applies to the players in the current study who were only consuming an average of 31.0 kcal·kg^-1·day^-1. Players in the present study would be advised to increase their relative carbohydrate intake in preference to following percentage value recommendations to compensate for their low energy intake. This would need to be done carefully particularly since the players are statistically in energy balance, however there are a range of high carbohydrate foods that could be used to replace the moderate carbohydrate/high fat foods that players reported in their diaries. This would enable carbohydrate intake to be increased without simultaneously increasing energy intake.

Strength and endurance requirements of soccer mean that players need increased dietary protein than their sedentary counterparts to support muscle protein synthesis and possibly act as an additional fuel supply (Lemon, 1994). In the present study protein intake (16.8 ± 2.1%) was significantly higher (p < 0.05) than the 12% intake recommended by Devlin and Williams, 1991. This in agreement with Clark et al., 2003 who also reported values higher than the DRI (1.4 ± 0.3 g·kg^-1·day^-1). The reported intake of the current players (1.2 g·kg^-1·day^-1) is in line with the recommended range of 1.2-1.7 g·kg^-1·day^-1 for elite and professional athletes (Maughan and Burke, 2002) and players should be encouraged to continue this practice.

In comparison to the recommendations made by Devlin and Williams, 1991 fat intake was significantly higher (p < 0.05) than the lower value (18%), but not significantly different (p > 0.05) to the upper end of this range (28%). The fat intakes of the players in the current investigation (28.8 ± 6.6%) is in accordance with the <30% recommendations for soccer players made by Ruiz et al., 2005, and slightly lower than those previously reported by Scott et al., 2003 and Clark et al., 2003 (29-33%). Furthermore, from a health perspective the percent of total fat consumption from saturated fat was 10.6 ± 3.3%, and the percent from unsaturated sources 13.6 ± 3.8%. This is just higher than the maximal intake of saturated fat (10%) recommended for the general population (COMA, 1991). Consequently, whilst current fat intakes meet recommendations these are towards the higher end for athletic performance and health and players should be advised that further increases in fat intake would be detrimental.

Despite the players in the current study being in statistical energy balance and consuming each of the macronutrients within previously published recommendations for athletic populations, there is scope for an alteration of the proportional intake of each which would have practical significance in enhancing recovery rates and facilitating players' ability to train and perform at a high intensity. Carbohydrate and protein are key nutrients for soccer performance and in the reported data both of these nutrients were at the lower end of recommended intakes and thus may be limiting both the performance and rate of recovery of these players. It is recommended, therefore, that daily intakes of carbohydrate and protein be increased to levels that would be more optimal for muscle glycogen resynthesis and protein resynthesis; alongside a concomitant decrease in fat intake that would be beneficial for health and performance.

Water
The observed fluid intake of 2466 ± 1351 ml·day^-1 is slightly below the baseline recommendation of 2500 ml·day^-1 (Maughan, 2000), however this value does not consider additional requirements to replace fluid losses during training and matches. Convertino et al., 1996 state that during exercise 600-1200ml fluid needs to be consumed per hour to replace sweat losses. The reported average daily training duration of 108.2 ± 35.9 minutes would therefore require a minimum of 1200 ml, suggesting a total daily requirement of ~3700 ml, although exact fluid needs are highly individual and depend upon intensity and duration of exercise, body size and composition, body surface area, individual sweat rates, clothing and environmental conditions (Broad et al., 1996, Maughan et al., 2004). The current data indicate a shortfall in daily fluid intake of approximately 1200 ml. This would have implications for performance since dehydration increases the thermal load of exercise and results in elevated heart rate (McGregor et al., 1999; Montain and Coyle, 1992) and earlier onset of fatigue due to an increased rate of glycogenolysis (Fallowfield et al., 1996; McGregor et al., 1999) and decreased performance of soccer skills (McGregor et al., 1999). To ensure adequate fluid replacement during and after exercise, players should consume prescribed volumes of fluid, calculated on an individual basis to replace body fluid losses, in preference to ad-libitum practices (Maughan et al., 2004; Wong et al., 1998). Furthermore, players in the current study did not report regular consumption of carbohydrate- electrolyte drinks post-exercise. This would be a further recommendation since the inclusion of energy substrate helps to maintain the desire to drink whilst initiating glycogen re-synthesis in the active muscle (Wong et al., 1998) and would be a simple and practical strategy to increase carbohydrate and fluid intakes in the players.

Micronutrients
Players reported Iron intakes of 12.1 ± 6.0 mg·day^-1 which is below (82% of RNI), but not significantly different (p > 0.05) from the RNI (COMA, 1991). These data are similar to previously reported intakes of 12.2-17.3 mg·day^-1 for female soccer players (Clark et al., 2003). Iron deficiency in female athletes appears to be common (Bean, 2003; Nutter, 1991; Tilgner and Schiller, 1991) and can be due to dietary intake and menstrual losses as well as iron losses in sweat, gastrointestinal and bladder blood loss, or haemaglobinuria from red blood cell damage in the plantar flasia during running (Tunstall-Pedoe, 1984). This said, without supporting haematological data, nutritional insufficiency alone does not indicate clinical iron deficiency. In addition to this, iron deficiency can occur even if the diet provides a sufficient amount (Iglesias-Gutierrez et al., 2005). Therefore ensuring daily intake is optimal will reduce the likelihood of such a condition arising, but should not be used as a marker per se. As a component of haemoglobin, iron is particularly important in the oxygen carrying capacity of an individual and deficiencies may therefore compromise aerobic capacity. Kang and Matsuo, 2004 recently observed that 4-weeks of Iron supplementation by elite female soccer players significantly increased body iron stores and prevented training-induced decreases in Haemoglobin concentration. Players in the present study may benefit from ensuring sufficient iron rich foods or iron supplementations are included in the diet to meet the DRI.

Calcium intake 840 ± 335 mg·day^-1 was above the RNI of 700 mg·day^-1 (COMA, 1991). Previous research has indicated that low calcium intake is not uncommon in female athletes who restrict calorie intake or follow a low fat diet (Tanaka et al., 1995). Since fat intakes of the players in the present study were at the upper end of athlete recommended values, calcium deficiency was not anticipated.

Methodological considerations
Due to the errors inherent of all dietary recall methods, the values for seven days dietary analysis may not be accurate for all the measured parameters and should not be taken as an exact reflection of long-term nutrient status. Bingham, 1987 suggested that seven days is sufficient to assess energy intake but other nutrients may need to be observed over a longer period of time. Although nutrient intake recorded over seven days is subject to a degree of variability, this measure of consumption is more reliable than an observation of a single day (Marr and Heady, 1986). Methods used for measuring food intake rely on the participants' ability to accurately record 'what' and 'how much' is eaten. Unfortunately self-reported dietary intake protocols are frequently biased towards underestimation of dietary intakes, which may explain the discrepancy between energy intake and expenditure observed in the present study. Goldberg et al., 1991 proposed that energy intake data can be used to assess the validity of a seven day record, by the identification of a minimum level of energy expenditure. It was proposed that energy intakes below 1.1 x BMR are not viable and therefore unlikely to represent true habitual intake. Utilising this, the minimum level of energy expenditure in the present study is 1911 kcals, which is higher than the mean reported intake of 1904 kcals, suggesting that underestimation of true dietary intake may have occurred.

• Female soccer players demonstrate a low energy intake in relation to predicted requirements, but were in energy balance in this study.
• Increased carbohydrate intake may be beneficial to both training and competition performance of elite female soccer players
• Fluid requirements should be addressed on an individual basis and matched to player requirements.
• The iron status of female soccer players may be compromised due to insufficient dietary intake to meet the DRV.