Table 1.  Primer sequences used in quantitative real time RT-PCR.

GenBank
Acc. No.

Gene name

PS forward 5´ to 3´

PS reverse 5´ to 3´

Amplicon size (bp)

AT

QT

NM 001101

ß-actin

CTA CGT CGC CCT GGA CTT CGA GC

GAT GGA GCC GCC GAT CCA CAC GG

385

60°C

79°C

NM 012495

AldolaseA

ATGCCCCACCCATACCCAGCACT

AGCAGCAGTTGGCGGTAGAAGCG

191

64°C

79°C

NM 002046

GAPDH

GTC TTC ACC ACC ATG GAG AAG G

TCA TGG ATG ACC TTG GCC AG

198

60°C

79°C

NM 001082477

IGF-1

AAG CCT ACA AAG TCA GCT CG

GGT CTT GTT TCC TGC ACT TC

166

58°C

79°C

NM 052807

IGF-1R

AAA ACC ATC GAT TCT GTG ACG

GGT TCT TCA GGA AGG ACA AGG

199

60°C

79°C

NM 012588

IGF-BP3

CGC TAC AAA GTT GAC TAT GAG

CGT CTT TCC CCT TGG T

292

60°C

79°C
Abbreviations: GenBank Acc. No.= GenBank Accession number, PS = primer sequence, AT = annealing temperature, QT = quantification temperature, bp = base pairs, GAPDH = glycerine aldehyde 3-phosphate dehydrogenase, IGF-1 = insulin-like growth factor-1, IGF-1R = IGF-1 receptor, IGF-BP3 = IGF-binding protein 3.