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IGF-1 GENE EXPRESSION IN RAT COLONIC MUCOSA AFTER DIFFERENT EXERCISE
VOLUMES
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Katja
Buehlmeyer1 ,
Frank Doering2, Hannelore Daniel3, Anatoli Petridou4, Vassilis Mougios4,
Thorsten Schulz1 and Horst Michna1 |
1Institute of Public Health Research, Technical University Munich,
Munich, Germany, 2Molecular Nutrition, Christian-Albrechts-University of
Kiel, Kiel, Germany, 3Molecular Nutrition, Technical University Munich,
Freising, Weihenstephan, Germany, 4Department of Physical Education and
Sport Science, Aristoteles University of Thessaloniki, Thessaloniki, Greece.
| Received |
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12 March 2007 |
| Accepted |
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27
June 2007 |
| Published |
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01
December 2007 |
©
Journal of Sports Science and Medicine (2007) 6, 434- 440
| ABSTRACT |
| The evidence is increasing for a close link between the insulin/insulin-like
growth factor (IGF) system and colon cancer prevention by physical
exercise. To reveal exercise-induced alterations in colon mucosa,
gene expression of IGF-1 and related genes and serum IGF-1 were investigated.
Twenty male Wistar rats performed a 12 week voluntary exercise program.
Nine rats served as the control group. Gene expression of IGF-1, IGF-1
receptor (IGF-1R) and IGF-binding protein 3 (IGF-BP3) were quantified
by real-time RT-PCR. Circulating IGF-1 was analyzed exercise volume-dependent.
Based on 3 distinguished groups with low (L-EX, <2629 m·night-1),
medium (M-EX, 3003-7458 m·night-1) and high exercise volume (H-EX,
>8314 m·night-1), we observed lower serum IGF-1 levels (P <
0.05) in all exercise groups as compared to the control group and
IGF-1 levels declined proportional to the increase in exercise volume.
A significant (p < 0.05) positive correlation was found between
IGF-1 concentration and body mass (r = 0.50) and a significant negative
correlation exists between body mass and exercise volume (r = -0.50).
Significant differences in colonic mRNA levels of IGF-1, IGF-1R and
IGF-BP3 could not be observed. Based on our data we propose that the
exercise as well as the body mass reduction leads to a decrease in
circulating IGF-1 and this might represent a prime link to colon cancer
prevention.
KEY
WORDS: Cancer
prevention, IGF-1R, IGF-BP3, real-time RT PCR, physical exercise.
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