Figure 1. Assessment of vascular and autonomic function using non-invasive measurement techniques. Ankle occlusion pressure (AOP) and post-occlusive reactive hyperemia (PORH) were evaluated with laser Doppler flowmetry (LDF) to assess peripheral microcirculatory function. Heart rate variability (HRV) was measured using the Polar system with a chest-strap heart rate sensor, providing an assessment of autonomic nervous system activity.