Table 2. Summary of the main findings regarding neuromuscular responses.
Study Format of play (SSG) Assessment timings Main findings
Ascondo et al., 2024 4v4 wheelchair basketball, 4×4 min bouts, 2 min rest, 28×15 m court 20 m sprints (S5 m, S20 m, S5–20 m) before SSG (T0) and after each bout (T1–T4) SCI players showed significant loss of sprint capacity: ↑ S20 m and S5–20 m times at T2 and T3 vs T0 (p < .05, ES = 0.28–0.35, small). SCI players consistently slower than Non-SCI across tests (p < .01, ES = 1.52–2.12, large). Non-SCI showed no significant decrements across bouts. Correlations in SCI group: total RPEresTL correlated with decline in S5 m (r = 0.72, p < .01) and S20 m (r = 0.66, p < .05) between T0–T4. No significant correlations in Non-SCI.
Baseri et al., 2022 3v3 + 4 support players, 4×4 min, 3-min rest, 18×20 m, ball-possession with coach encouragement 20 m sprint at baseline and 48 h post No significant differences between recovery strategies for 20 m sprint (F = 0.361, p = 0.78, η2 = 0.02). Within-condition analysis showed significant improvement from baseline to 48 h (p = 0.001, η2 = 0.37, moderate effect).
Bekris et al., 2022 3v3, 8×3 min, 3-min rest, 20×25 m, no GK, ball possession Squat jump, 20 m sprint at PRE, POST, 24 h, 48 h, 72 h No significant impairments in squat jump (F(1.68,18.45)=1.40, p ≥ 0.05, η2=0.11) or 20 m sprint (F(2.02,22.20)=2.65, p ≥ 0.05, η2=0.19). Performance remained stable across time points.
Clemente et al., 2017 1v1 (3×2 min) and 3v3 (3×3 min), 1:1.5 work-to-rest Squat jump (SJ) and countermovement jump (CMJ) before, after each bout No significant differences across bouts in SJ (p = 0.981, ES = 0.415, moderate) or CMJ (p = 0.307, ES = 0.112, minimum). No significant interaction with format. Neuromuscular output not impaired despite increased HR and RPE.
Dello Iacono et al., 2016 3v3 handball, 5×3 min bouts, 1-min rest, with vs without contact CMJ and plyometric press-up before and immediately after SSG Contact SSG (C-SSG): Significant impairments in CMJ: Fpeak-ecc -6.4% (p<0.001, η2=0.979), Secc -3.3% (p<0.001, η2=0.961), Fpeak-con -2.4% (p<0.001, η2=0.992), Ppeak-con -3.2% (p<0.001, η2=0.995), JH -5.2% (p=0.001, η2=0.381). Upper-body PP: Fpeak-ecc -9.6% (p<0.001, η2=0.989), Fpeak-con -6.4% (p<0.001, η2=0.989), Ppeak-con -11.5% (p<0.001, η2=0.911). No-contact SSG (NC-SSG): Small improvements: CMJ JH +2.5% (p<0.05), Ppeak-con +8.1% (p<0.001); PP Fpeak-con +2.3% (p<0.05).
Iturricastillo et al., 2018 4v4 WB, 4×4 min bouts, 2-min passive recovery 5 m & 20 m sprints, 5 m & 20 m sled towing, CODA pre vs post Sprint performance declined 1.10% at 5 m and 20 m (p < .01; ES ≤ 0.14). Sled towing declined 1.82% at 5 m (p < .05; ES = 0.18) and 2.68% at 20 m (p < .01; ES = 0.27). CODA showed trivial change (-0.41%, p > .05, ES = -0.04). Δ% LS–ST significantly ↑ post at 20 m (p < .05, ES = 0.38), but not 5 m (p > .05, ES = 0.06). Negative correlations: Δ% sprint (5 m r = -0.42; 20 m r = -0.55, p < .01) with Δ% blood lactate; Δ% sled towing (20 m) with Δ% tympanic temperature (r = -0.45) and lactate (r = -0.46).
Johnston et al., 2014 Rugby league, “offside” 6v6 SSG (2×8 min halves, 70×30 m), with vs without contact (16 × 10 s bouts/half) CMJ and plyometric press-up PRE, POST, 12 h, 24 h CMJ (lower body): Reductions POST in both games: contact ES = -0.88; non-contact ES = -1.42. At 12 h: contact ES = -1.40; non-contact ES = -2.25. At 24 h: contact ES = -0.35 vs non-contact ES = -1.13. Differences between games were practically meaningful (likelihood ≥75–92%). PP (upper body): No change in non-contact. Contact game → large reduction POST (ES = -1.86), moderate reductions at 12 h (ES = -0.74) and 24 h (ES = -0.74). Between-group differences: POST (ES = -1.31, almost certain), 12 h (ES = -0.68, very likely), 24 h (ES = -0.87, likely).
Madison et al., 2019 3v3 (20×15 m, 300 m2, ~50 m2/player) vs 4v4 (40×25 m, 1000 m2, ~125 m2/player), 6×4 min, 90 s rest Isometric hamstring torque (NordBord) at 90° and 30° knee flexion, pre vs post Larger-area 4v4 SSG induced greater decrements. Peak hamstring force at 90°: -13.62 N vs -5.78 N in 3v3 (p<0.05, d=0.60). Mean hamstring force at 90°: -24.78 N vs -11.11 N (p<0.05, d=0.51). Relationship between total accelerations and peak torque decrement at 90°: r=0.46, p=0.039. Interpretation: higher external loads in larger SSGs produce greater hamstring fatigue.
Martínez-Serrano et al., 2023 4v4 + 3 floaters SSG on MD-4 (~activation microcycle), followed by TR1 (6v6 + 2 GK on 40×40 m and 10v8 transitions in ¾ pitch) and TR2 (7v7 + GK on 90×60 m and 10v8 transitions in ¾ pitch) Isometric MVIC of knee extensors and posterior chain: baseline, post-ACT, post-SSG, post-TR1, post-6h, post-24h, post-PREV, post-TR2 Posterior chain (90:20 MVIC): significant ↓ in “HIGH” HSR group after TR1 (-14.08%, 337.7 ± 105.8 N → 290.2 ± 104.2 N, p = 0.037, ES = 0.45). Recovery incomplete at 6h (-9.4%) and 24h (-5.0%) vs baseline. “LOW” HSR group showed no impairment (Δ = +0.2 to +6.5%). Knee extensor MVIC unaffected by training load (F = 1.155, p = 0.332).
Modena & Schena, 2024 3v3 (30×20 m, 100 m2/player) vs 6v6 (60×40 m, 200 m2/player), 4×4 min bouts, 2-min rest, no GK Sprint (10, 20, 30 m) and CMJ pre, post, 24 h Sprint: Both formats impaired sprint performance post (10 m: +0.02–0.04 s, p<0.05; 20 m: +0.04–0.05 s, p<0.01; 30 m: +0.07–0.08 s, p<0.001). At 24 h, 3v3 recovered to baseline (no sig. diffs), but 6v6 remained slower at 30 m (Δ+0.03 s vs pre, p=0.021, g=-0.29). CMJ: 3v3: trivial ↑ at 24 h (35.9 → 38.4 cm, p=0.001, g=0.40). 6v6: ↓ post (37.9 → 36.3 cm, p=0.038, g=-0.28), remained ↓ at 24 h (36.1 cm, p=0.021, g=-0.29).
Mora et al., 2025 7v7 + 2 GK, 3×8 min (LSG8, 68×40 m, 194 m2/player, 5-min rest) vs 6×4 min (LSG4, 68×40 m, 194 m2/player, 2-min rest) vs 6×4 min (SSG4, 40×34 m, 97 m2/player, 2-min rest) CMJ, kick velocity (KV), 20-m sprint (split 0–10 m, 10–20 m) pre vs post CMJ: No sig. changes post in any format (LSG8 ES = -0.16; LSG4 ES = -0.10; SSG4 ES = -0.06; all p > 0.05). KV: Small ↓ after SSG4 (p = 0.04, ES = -0.54). Sprint: All formats impaired sprint performance. 0–10 m: small impairment after LSG4 (p < 0.01, ES = 0.29). 10–20 m and 0–20 m: moderate-to-large impairments after all formats (p = 0.00–0.01, ES = 0.60–1.50). Greater 0–10 m impairment observed in LSG4 vs LSG8 (p = 0.03).
Papanikolaou et al., 2021 4v4 (20×25 m, 62.5 m2/player, 6×4 min, 180-s rest) vs 8v8 (70×65 m, 284.4 m2/player, 3×8 min, 90-s rest), no GK MVIC KE/KF at baseline, 1h, 2h, 3h; isokinetic concentric/eccentric strength, CMJ, 30-m sprint at baseline, 24h, 48h, 72h MVIC: ↓ KE + KF at 1h–2h in both formats; extended to 3h in 8v8. Isokinetic strength: KE concentric ↓ 24h in both formats; KE eccentric ↓ up to 72h in 8v8 (p<0.05). KF concentric ↓ 24h; KF eccentric ↓ 24–72h (p<0.05). CMJ: ↓ 24h in 4v4; ↓ post, 24h, 48h in 8v8. Sprint: ↓ 24–48h in 4v4; ↓ 24–72h in 8v8.
Pellegrino et al., 2020 4v4, U18 elite males, 8-min bouts, pitches 125, 150, 250, 300 m2; pre and post repeated-sprint ability (RSA) test (10×40 m shuttles, 25 s recovery) External load from GPS (sprints, accelerations, distances, Vmax, Vmean) pre vs post RSA 125 m2: Post-RSA decrements: Vmax -1.81 km/h (CI -3.05 to -0.57, ES=0.97, p=0.005), Vmean -0.69 km/h (CI -1.16 to -0.23, ES=1.26, p=0.004), sprint number -6.56 (CI -10.13 to -3.00, ES=1.13, p<0.001), accelerations zone 2 -2.69 (CI -5.13 to -0.24, ES=0.68, p=0.032), sprint distance -65.44 m (CI -103.73 to -27.16, ES=1.20, p=0.001). 150 m2: Distance ↓ -88.3 m (ES=0.95, p=0.018). 250–300 m2: Larger pitches allowed higher high-intensity running; post-RSA, Vmean ↓ in 300 m2 (-0.88 km/h, ES=1.17, p<0.001), sprints ↓ in 250 m2 (-3.69, ES=0.72, p=0.043). Overall, fatigue impaired high-intensity actions most in the smallest pitch (125 m2).
Sjökvist et al., 2011 4v4 SSG (32×22.5 m, 4×4 min, 3-min active recovery) + soccer-specific interval running (4×4 min, 3-min recovery), with and without the ball CMJ, 5BT, 20-m sprint pre, 24 h, 48 h, 72 h post CMJ: ↓ after 24 h vs baseline (48.8 ± 7.9 → 46.9 ± 7.6 cm, p<0.04), recovered by 48 h (48.7 ± 7.9) and 72 h (49.3 ± 8.3). 5BT, 20SP: No sig. changes across recovery intervals (p>0.05).
Skala & Zemková, 2023 4v4 + GK, 40×25 m pitch (125 m2/player), 6×4 min bouts, 1-min rest CMJ, planned agility (PA), reactive agility (RA) pre vs post CMJ: ↓ -6.65% (29.7 → 27.7 cm, p=0.014, g=0.56). PA: ↑ +4.04% (1.98 → 2.06 s, p=0.002, g=0.97). RA: ↑ +6.45% (2.17 → 2.31 s, p=0.003, g=1.16). Fatigue impaired explosive strength and agility.
Sparkes et al., 2018 4v4 + GK, 6×7 min bouts, 2-min rest, 24×29 m pitch CMJ (PPO, JH) pre, 0h, +2h, +24h PPO: ↓ at 0h (-1.1 W·kg-1, ±0.9, possibly small), recovered at +2h (+0.7, trivial), ↓ again at +24h (-0.9, ±0.8, small). JH: ↓ at 0h (-3.2 cm, ±1.9, moderate), recovered at +2h (+0.1 cm, trivial), ↓ again at +24h (-2.5 cm, ±1.2, small). Shows bimodal recovery pattern.
Sparkes et al., 2020a 4v4 + GK, 6×7 min, 2-min rest, 24×29 m pitch, compared across 2 sessions CMJ (PPO, JH) pre, 0h, 24h PPO: Session 1 ↓ 0h (-0.9 W·kg-1, ±0.8, small), recovered at 24h (-0.3, ±0.6, trivial). Session 2 ↓ 0h (-0.9, ±0.8, small), ↓ at 24h (-0.9, ±0.6, small). JH: Session 1 ↓ 0h (-3.4 cm, ±2.2, moderate), recovered 24h (-0.4, ±2.0, trivial). Session 2 ↓ 0h (-3.3, ±2.2, moderate), ↓ 24h (-1.9, ±2.0, small). Findings suggest greater fatigue persistence when players were in congested periods .
Sparkes et al., 2020b 4v4 + GK, 6×7 min bouts, 2-min rest, 24×29 m pitch; compared two orders: (a) SSG → resistance (SSG+RES), (b) resistance → SSG (RES+SSG), 2-h interval CMJ (JH, PPO) pre, 0h, +24h JH: RES+SSG ↓ 0h (-4.1 ± 2.6 cm, p<0.001, d=0.67, moderate), recovered +24h (-1.3 ± 2.0 cm, ns). SSG+RES ↓ 0h (-2.2 ± 3.1 cm, p=0.061), residual at +24h (-2.6 ± 4.9 cm, ns). PPO: RES+SSG ↓ 0h (-3.53 ± 2.48 W·kg-1, p<0.001, d=0.50), recovered +24h (-1.56 ± 2.30, ns). SSG+RES minimal change (-0.84 ± 2.75, ns). Significant protocol × time interaction for PPO (p=0.009).
Sparkes et al., 2022 4v4 + GK, 6×7 min, 2-min rest, 24×29 m pitch, repeated on consecutive weeks CMJ (JH, PPO) pre vs post Reliability: Pre SSG ICC very high for PPO (0.95, CV=2.1%), high for JH (0.82, CV=3.9%). Response consistency: Pre→post change ICC moderate (PPO 0.68; JH 0.77). JH ↓ -1.5 to -1.8 cm across weeks; PPO ↓ -41 to -80 W.
Trecroci et al., 2019 Postmatch interventions: (a) Soccer-specific training (SST: 4×3-min 4v4 SSG, tactical drills, set plays, ~60 min); (b) Active recovery (AR: low-intensity circle drills + jogging, ~30 min) Assessments: PRE (-72 h), Postmatch (0 h), Postintervention (+72 h) 30-m sprint: Impaired 0 h (↑ time, ES=1.3–2.4), recovered by +72 h under both SST (-4.47 ± 0.21 → 4.28 ± 0.13 s, p=0.005) and AR (4.51 ± 0.15 → 4.28 ± 0.22 s, p=0.016). RSA (5×30 m): Impaired 0 h (ES=0.8), recovered by +72 h under both interventions (p<0.01). MVF knee extensors: Impaired 0 h (ES=0.8–1.3), recovered similarly in both SST (+7.2%, p=0.045) and AR (+11.1%, p=0.004). MVF knee flexors: Impaired 0 h (ES=1.0–2.0). Recovered only under AR (+25.7%, p=0.001); no sig. recovery in SST (+9.4%, p=0.083). Significant time×intervention interaction (p=0.036). AR > SST in restoring hamstring strength (ES=-0.60).
CMJ: countermovement jump; SJ: squat jump; JH: jump height; PPO: peak power output; MVF: maximal voluntary force; MVIC: maximal voluntary isometric contraction; KE: knee extensors; KF: knee flexors; RSA: repeated-sprint ability; 5BT: five-bound test; 20SP: 20-m sprint performance; PA: planned agility; RA: reactive agility; PP: plyometric press-up; Fpeak-ecc: peak eccentric force; Secc: eccentric impulse; Fpeak-con: peak concentric force; Ppeak-con: peak concentric power; CODA: change-of-direction ability; KV: kick velocity; S5 m: 5-m sprint time; S20 m: 20-m sprint time; S5–20 m: flying 5–20 m sprint segment; LSG: large-sided game; SSG: small-sided game; GK: goalkeeper; WB: wheelchair basketball; SCI: spinal cord injury; Non-SCI: non–spinal-cord-injured.