Table 3. Summary of the main findings regarding physiological responses.
Study Format of play (SSG) Assessment timings Main findings
Alashti et al., 2021 2v2 (8×2 min / 1 min rest, 20×25 m), 4v4 (4×4 min / 2 min rest, 28×35 m), no GK, stop-ball rule HR measured pre, 2nd, 8th, 14th, 20th min, and immediately post HR significantly increased in both SSGs compared with control (p ≤ 0.05). Example: in 2v2, HR rose from 71.27 ± 2.5 bpm pre to 187.51 ± 3.20 bpm at 20th min (~90% HRmax). In 4v4, HR increased from 69.09 ± 2.32 bpm pre to 182.45 ± 8.11 bpm at 20th min (~89% HRmax). Larger SSG (4v4) elicited lower HR/%HRmax compared to 2v2.
Ascondo et al., 2024 4v4 wheelchair basketball, 4×4 min bouts, 2 min rest HR continuously during bouts; tympanic temp, RPEres, RPEmus after each bout (S1–S4) Non-SCI group had higher HRpeak and HRmean than SCI across all bouts (p < .05–.01, ES = 0.73–1.39, moderate-large). SCI reported higher muscular perceived load in S1–S2 (p < .05, ES = 0.75–0.82, moderate) and higher respiratory perceived load in S4 (p < .05, ES = 0.97). Within-group changes: SCI showed stable HR and Temp across bouts; Non-SCI showed ↑ HRmean (S2, S3 vs S1, p < .05, ES = 0.29–0.57), ↑ HRpeak (S3, S4 vs S1, p < .05, ES = 0.42–0.65), ↑ RPEmusTL (S2–S4 vs S1, p < .05–.01, ES = 0.29–0.70). Temp increased S4 vs S1 for pooled players (p < .01, ES = 0.28, small).
Baseri et al., 2022 3v3 + 4 support players HRV (SDNN, lnRMSSD) pre-SSG, post-SSG, post-recovery (24 h, 48 h) Significant differences in SDNN HRV between AR vs. CWI (F = 4.86, p = 0.03, η2 = 0.31, moderate). CWI better restored vagal-related HRV: mean difference +23.2, +242%. Within-condition changes (F = 60.82, p = 0.001, η2 = 0.85, strong) confirmed significant drops from pre to post-SSG (p = 0.001) and recovery improvements (p = 0.001). lnRMSSD also differed between AR vs. CWI (F = 2.41, p = 0.033, η2 = 0.29); CWI showed greater effect (mean difference +0.81, +185.7%).
Bekris et al., 2022 3v3 HR continuously across 8 sets; RPE PRE, during sets 2 & 5, POST, and 24–72 h; blood lactate PRE, after set 2, set 5, and POST HR reached 168 ± 7 bpm (~87 ± 4% HRmax), peaking at 92 ± 3% HRmax after the 8th set (F(1.00,11.04)=5647.93, p < 0.001, η2=1.00). Blood lactate increased significantly: 11.13 ± 2.23 mmol/L (after set 2), 10.79 ± 2.24 mmol/L (set 5), 13.02 ± 1.60 mmol/L POST (F(1.81,19.97)=44.06, p < 0.001, η2=0.80). RPE progressively increased, peaking POST (~15 ± 1, Borg scale) (F(3.43,37.76)=292.98, p < 0.001, η2=0.96), returning to baseline by 72 h.
Bonato et al., 2020 4v4, 4×4 min, 3-min rest, 36×24 m, no GK, evening session (20:00) HR monitored continuously HRmean across bouts: 172 bpm (90% HRmax), 174 bpm (91%), 178 bpm (94%), 173 bpm (91%). No significant differences compared to HIIT.
Clemente et al., 2017 1v1 (3×2 min) and 3v3 (3×3 min) HR monitored across bouts; RPE pre and post bouts HRaverage differed between bouts (p = 0.026, ES = 0.306, minimum). Bout 3 > Bout 2 (p = 0.027). %HRmax also higher in Bout 3 vs Bout 2 (p = 0.026, ES = 0.313). No differences between formats (p = 0.953). RPE increased significantly across bouts (p = 0.001, ES = 0.843).
Dellal et al., 2015 2v2 (4×2 min, 20×15 m), 3v3 (4×3 min, 25×18 m), 4v4 (4×4 min, 30×20 m), with/without GK; 3-min passive recovery HRR measured at 1, 2, 3 min post-exercise (beats lost/min) SSGs: HRR1 greater in 4v4 w/ GK, 3v3 w/ GK, and 4v4 vs 2v2 (F(5,126)=6.26, p<0.01). HRR2 greater in 2v2 w/ GK, 2v2, 3v3 vs 4v4 (F(5,126)=4.51, p<0.01). HRR3 lower in 3v3 w/ GK vs other SSGs (F(5,126)=7.09, p<0.01). End-exercise HR: 2v2 = 186.4 bpm (89% HRmax), 2v2+GK = 183.7 (87.7%), 3v3 = 183.4 (87.5%), 3v3+GK = 180.2 (86.0%), 4v4 = 178.3 (85.1%), 4v4+GK = 173.6 (82.9%).
Dello Iacono et al., 2016 3v3 handball HR during bouts No differences between C-SSG and NC-SSG in %HRmean (85.6 vs 86.2%) and HRmax (192 vs 193 bpm).
Iturricastillo et al., 2018 4v4 WB, 4×4 min Tympanic temperature pre vs post; HRmean, HRpeak, Edwards’ TL, TRIMPMOD, RPEres, RPEmus across bouts; Capillary blood lactate pre vs post Tympanic temperature ↑ from 36.21 ± 0.60 °C to 36.97 ± 0.59 °C (Δ = +2.11%, p < .001, ES = 1.27). Internal load: HRmean 156 ± 11 bpm, HRpeak 179 ± 13 bpm. Edwards’ TL = 67.5 ± 6.7 AU; TRIMPMOD = 55.3 ± 12.5 AU; RPEres TL = 100.6 ± 25.9 AU; RPEmus TL = 102.3 ± 29.7 AU. Negative associations: Δ% sled towing correlated with Δ% temperature (r = -0.45, p < .01). Lactate ↑ from 1.95 ± 1.30 mmol/L to 5.84 ± 2.04 mmol/L (Δ = +199.5%, p < .001, ES = 2.99). Higher lactate changes correlated with smaller declines in sprint and sled towing (r = -0.42 to -0.55, p < .01).
Johnston et al., 2014 Rugby league, “offside” 6v6 SSG (2×8 min halves, 70×30 m), with vs without contact (16 × 10 s bouts/half) Session RPE 30 min post; wellness scale PRE, POST, 12 h, 24 h RPE higher in contact (6.9 ± 0.4) vs non-contact (6.3 ± 0.6), p = 0.05, ES = 0.41 (small). Wellbeing: greater reductions after contact (F(1,22)=10.88, p=0.03, η2=0.338). Muscle soreness moderately higher after contact (ES = 0.71).
Karadağ et al., 2024 2v2 (20×25 m, 3×4 min, 3-min rest), 4v4 (30×35 m, 3×4 min), 8v8 (40×45 m, 3×4 min) HR measured pre, immediately post, and 30 min post Intragroup: MHR significantly ↑ post vs pre (p<0.05) and ↓ at 30 min vs post (p<0.05), but remained above pre (p<0.05). Example: 2v2 → 63.0 ± 3.46 (pre) → 181.5 ± 3.00 (post) → 78.0 ± 4.89 (30 min). 4v4 → 68.25 ± 4.46 → 175.25 ± 20.05 → 87.0 ± 11.56. 8v8 → 66.5 ± 5.77 → 125.7 ± 18.41 → 88.5 ± 9.33. Intergroup: Post values higher in 2v2 & 4v4 vs 8v8 (p<0.05). No differences pre or 30 min post between formats.
Kryściak et al., 2023 1v1, 6×30 s (E1) vs 6×45 s (E2), 1:4 work-to-rest, 10×15 m pitch, side-boards, no GK HR (%HRmax) measured at rest (T0), after each bout (T1–T6), +15 min (T7), +30 min (T8) %HRmax: time effect (F(8,144)=1252.84, p≤0.0001, η2=0.99). Mean 86.7–90.8% across bouts. No group effect during SSGs (F(1,18)=3.85, p=0.065, η2=0.18). Post-exercise recovery faster in 45 s SSGs: at T7 (57.2% vs 63.5%, p=0.021, d=1.01) and T8 (52.4% vs 60.1%, p=0.043, d=0.87).
Madison et al., 2019 3v3 vs 4v4, as above HR monitored continuously Large SSGs showed higher internal load: HRmean 163 ± 16 bpm vs 157 ± 25 bpm (p=0.003, d=0.26), HRmax 194 ± 13 vs 188 ± 28 bpm (p=0.001, d=0.27). GPS: greater distance in high-speed zones, max speed (26.1 ± 2.0 vs 23.7 ± 1.8 km/h, p=0.001, d=1.29), and metabolic power (8.7 ± 0.9 vs 8.1 ± 1 W/kg, p=0.003, d=0.38) in large vs small SSG.
Martínez-Serrano et al., 2023 4v4 + 3 floaters SSG, TR1 (6v6+GK, 10v8 transitions), TR2 (7v7+GK, 10v8 transitions) RPE after TR1 and TR2 No significant differences between “HIGH” and “LOW” HSR groups: TR1 RPE 6.82 ± 1.29 vs 6.00 ± 1.46 (p = 0.637, ES = 0.58). TR2 RPE 6.71 ± 1.38 vs 6.00 ± 1.60 (p = 0.109, ES = 0.62). Suggests RPE underestimated localized neuromuscular fatigue.
Mascarin et al., 2018 4v4, 4×4 min, 3 min rest, ~120 m2/player HRV (LF, HF, LF/HF, RMSSD, pNN50, SDNN) pre, 10 min, 24 h, 48 h, 72 h 10 min post: LF ↑ +92.5% (very likely), HF ↓ -65.7% (very likely), LF/HF ↑ +386.2% (very likely). RMSSD ↓ -61.4% (very likely), pNN50 ↓ -90% (very likely). SDNN trivial (-13.5%). By 24 h, indices returned near baseline; 48–72 h showed parasympathetic rebound (HF, RMSSD, pNN50 ↑; LF, LF/HF ↓).
McLean et al., 2016 3v3, 6×2 min bouts, 15×20 m pitch, no goals/GK, unlimited touches; recovery = 30 s (REC-30) vs 120 s (REC-120) Vastus lateralis oxygenation (HHb, O2,Hb, tHb), HR, RPE during bouts and recovery HHb: Higher during recovery in REC-30 vs REC-120 (p<0.001, η2=0.725). No differences during bouts. HR: Higher during recovery in REC-30 vs REC-120 (p=0.001, η2=0.849). HR during bouts ~80–90% HRmax in both conditions; no condition effect (p=0.295). RPE: Increased across bouts (p<0.001, η2=0.610). Condition × bout interaction (p=0.016): REC-30 showed earlier increases (from B1→B4, B5, B6, p<0.01). Time-motion (GPS): No differences between conditions in total distance (REC-30: 1365 ± 37.7 m vs REC-120: 1347 ± 37.7 m, p=0.638) or % time in speed zones (all p>0.05).
Mitrotasios et al., 2021 4v4 + 2 GK, 6×4 min, 30×20 m HR during games Mean HR ~89% HRmax, replicating competitive demands.
Modena & Schena, 2024 3v3 vs 6v6, 4×4 min RPE post; DOMS pre, post, 24 h; TQR pre, 24 h RPE: No differences between formats (3v3: 5.5 ± 1.5 vs 6v6: 5.3 ± 0.7, p=0.328, trivial). DOMS: ↑ in both formats post (3v3: 17.7 → 33.2 mm, p=0.002; 6v6: 14.8 → 33.6 mm, p<0.001) and remained ↑ at 24 h (3v3: 25.4 mm, p=0.040; 6v6: 24.1 mm, p=0.015). TQR: ↓ at 24 h vs pre (3v3: 16.1 → 14.1, p=0.013, g=-0.75; 6v6: 16.2 → 13.6, p=0.002, g=-1.21).
Mora et al., 2025 7v7 + 2 GK, same as above GPS (TD, LIR, HIR, VHIR, Sprinting, Vmean, Vmax, Acc, Dec) across 3×8-min periods Pitch size effect: LSG4 > SSG4 for TD (2709 ± 228 vs 2252 ± 156 m), HIR (295 ± 68 vs 150 ± 44 m), VHIR (164 ± 47 vs 55 ± 33 m), Sprinting (125 ± 69 vs 18 ± 23 m), Vmean (113 ± 10 vs 94 ± 7 m/min), Vmax (25.2 ± 2.1 vs 21.4 ± 1.9 km/h), all p < 0.05. Bout duration effect: LSG4 vs LSG8: LSG4 showed higher VHIR (164 ± 47 vs 118 ± 45 m, p = 0.01). Time effects: LSG8 showed decrements in TD, LIR, Vmean across bouts 2–3 vs bout 1 (p < 0.05). LSG4 only dropped in bout 3. SSG4 remained constant across bouts. No sig. differences in accelerations or decelerations among formats (p > 0.05).
Papanikolaou et al., 2021 4v4 vs 8v8 (as above) HR, RPE, blood lactate post HR: Mean %HRmax higher in 4v4 (88.7 ± 6.6%) vs 8v8 (81.5 ± 8.7%, p=0.024). Peak HR 93.8% vs 88.6% HRmax (p=0.047). Time >90%HRmax: 14.6 ± 8.3 s vs 7.7 ± 8.2 s (p=0.027). RPE: Higher in 8v8 (8.7 ± 1.2) vs 4v4 (6.2 ± 1.1, p=0.000). Lactate: ↑ post in both, higher in 4v4 (p=0.002).
Pellegrino et al., 2020 4v4, 125–300 m2, pre/post RSA Distances covered in locomotor zones (0–21+ km/h) 125 m2: Post-RSA, distance in 7–14 km/h zone ↓ -129.98 m (CI -193.49 to -66.46, ES=1.38, p<0.001). 250–300 m2: Compared with 125–150 m2, players covered significantly more high-intensity distance (>18 km/h): pre (250 vs 125: +48.3 m, ES=2.03; 300 vs 125: +45.5 m, ES=2.27), post (250 vs 125: +65.6 m, ES=2.58; 300 vs 125: +70.3 m, ES=3.15), all p<0.05.
Ravier & Marcel-Millet, 2020 3v3 handball SSG with GK, intermittent 30 s play/30 s rest, 2×8 min, 2-min rest between halves HR (mean, peak, end), HRR30, HRR60, HRR300, HRR600; HRV (lnRMSSD, lnSDNN, lnSD1, lnHF, lnLF) pre (10 min) vs post (10 min seated recovery) HR: HRmean 164.9 ± 8.3 bpm (85.7 ± 3.7% HRmax), HRpeak 179.9 ± 8.3, HRend 171.1 ± 7.6. Higher than RS (HRmean 146.4) and CT (129.2). HRR: SSG HRR30 = 27.6 bpm, HRR60 = 50.0 bpm, nHRR60 = 29.2%. RS > SSG in HRR30/60 (p < 0.05–0.001). HRV: lnRMSSD ↓ from 4.10 ± 0.37 → 2.94 ± 0.69 (p < 0.0001, large ES). lnSDNN ↓ 4.37 ± 0.30 → 3.54 ± 0.35 (p < 0.0001). lnSD1 ↓ 3.75 ± 0.37 → 2.59 ± 0.71 (p < 0.0001). lnHF ↓ 3.59 ± 0.30 → 3.10 ± 0.77 (p < 0.01). Parasympathetic disruption greater than RS (lnRMSSD 2.94 vs 3.38, p < 0.01) and CT (3.65, p < 0.001).
Sansone et al., 2019 3v3 basketball, half-court (14×15 m), offense vs defense, long-intermittent (3×4 min, 2′ rest) vs short-intermittent (6×2 min, 1′ rest) External load (PlayerLoad), %HRmax, Edwards’ TL during games PlayerLoad: Offense > defense (148.0 ± 16.8 vs 137.1 ± 15.5 AU, p=0.008, η2=0.517). Short > long regime (147.0 ± 18.2 vs 137.9 ± 14.6 AU, p=0.026, η2=0.404). %HRmax: Offense > defense (91.1 ± 4.1% vs 88.7 ± 5.4%, p=0.020, η2=0.433). No regime effect (90.0 ± 5.6 vs 89.8 ± 4.2%, p=0.893). Strong task × regime interaction (p=0.002, η2=0.632). Edwards’ TL: Offense-long > defense-short (56.6 ± 2.4 vs 52.4 ± 4.4 AU, p=0.004, r=0.25).
Sjökvist et al., 2011 4v4 SSG + interval running, as above HR response (mean HR, %HRmax, time in HR zones), session-RPE HR: Mean session HR = 76.7 ± 4.3% HRmax. Players spent 23.4 ± 1.1 min >90% HRmax. No differences in HR responses across recovery intervals. S-RPE: ↑ after 24 h vs baseline (7.9 ± 0.4 → 8.4 ± 0.5, p<0.02), recovered by 48 h and 72 h.
Skala & Zemková, 2023 4v4 + GK, as above HR, GPS load, fatigue VAS; Go/no-go task (GNG) pre vs post HR: HRavg 171.7 ± 7.1 bpm (86.6% HRmax), time >90%HRmax = 45.9% of SSG. External load: TD 2753 m (91.8 m/min); HSR 379 m; VHSR 13.5 m; MSP 22.3 km/h. Fatigue VAS: ↑ +41.6 AU (p<0.001, g=4.15). Response time (GNGt): -3.36% (ns, p=0.119). Errors (GNGe): ↑ +87.1% (0.93 → 1.71 errors, p=0.023, r=0.57).
Sparkes et al., 2020a 4v4 + GK Mood disturbance questionnaire (BAM+) pre, 0h, 24h Session 1: Mood ↑ 0h (+14.4 AU, ±5.3, moderate), recovered 24h. Session 2: Mood ↑ 0h (+12.8 AU, ±4.7, moderate), remained ↑ 24h (+5.2 AU, ±3.8, small).
Sparkes et al., 2020b 4v4 + GK, same as above Mood (BAM+) pre, 0h, +24h Mood disturbance: ↑ at 0h after SSG+RES (+8.6 ± 9.1 AU, p=0.011, d=0.72), recovered at +24h. RES+SSG: no sig. mood change (pre → 0h +3.2 ± 11.4 AU, ns). No differences between protocols at +24h.
Sparkes et al., 2022 4v4 + GK, same format repeated 1 week apart GPS (TD, MSR, HSR, MV, PlayerLoad, HI Acc, HI Dec) Repeatability: TD ICC=0.63, CV=5.9%; MV ICC=0.55, CV=4.4%; PlayerLoad ICC=0.70, CV=7.5%. Poor repeatability in MSR (CV=22.1%), HSR (CV=62.4%), HI Dec (ICC=0.30, CV=29.0%). HI Acc ICC=0.81 (high).
Trecroci et al., 2019 SST vs AR, as above Internal load (RPE), GPS (TD, metabolic power, HR, accelerations) SST vs AR: Higher demands in SST (RPE 3.6 ± 1.2 vs 1.1 ± 0.6, p<0.0001; TD 4.25 ± 0.51 vs 1.88 ± 0.33 km; metabolic power 3.74 ± 0.67 vs 1.90 ± 0.36 W·kg-1; HR >75% HRmax: 1103 s vs 102 s). Distances at accelerations/decelerations >1 m·s-2; also higher in SST (all p<0.0001).
Trecroci et al., 2021 SST vs AR GPS, HR, RPE during interventions; Muscle soreness (VAS), Total Quality Recovery (TQR) at -72 h, 0 h, +72 h SST: RPE 3.6 ±1.1 AU vs AR 1.1 ±0.4 (p<0.001). Total distance SST 4.1 ±0.4 km vs AR 1.8 ±0.3. HR>85%HRmax: 270 s SST vs 0 s AR. Acc/Dec 2–3 m·s-2;: 102 ±38 m /133 ±42 m in SST vs 0 m AR. Confirms much higher physiological load in SST. Muscle soreness: Significant interaction (F(1,16)=7.901, p=0.004, η2p=0.497). AR ↓ soreness more from 0h→+72h (5.00 ±0.82 → 1.83 ±0.96 AU, p<0.0001, ES=4.2) vs SST (5.22 ±0.83 → 3.61 ±0.61 AU, p=0.033, ES=2.2). TQR: No sig. differences (baseline 16.3 ±1.7 → +72h 15.4 ±1.4 SST; 16.4 ±1.7 → 16.5 ±1.1 AR, p>0.05).
HR: heart rate; HRmax: maximal heart rate; HRmean: mean heart rate; HRpeak: peak heart rate; HRend: end-exercise heart rate; HRR: heart-rate recovery; HRR1/HRR2/HRR3: beats recovered in minutes 1/2/3 post-exercise; HRR30/HRR60/HRR300/HRR600: heart-rate recovery at 30/60/300/600 s; nHRR60: normalized HRR at 60 s; RPE: rating of perceived exertion; S-RPE: session RPE; TQR: total quality of recovery; DOMS: delayed-onset muscle soreness; VAS: visual analogue scale; BAM+: Brief Assessment of Mood; PlayerLoad: accelerometer-derived external-load metric (arbitrary units); TD: total distance; Vmean: mean speed; Vmax: maximum speed; MSP: maximal sprinting speed; HIR: high-intensity running; VHIR: very high-intensity running; HSR: high-speed running; VHSR: very high-speed running; Sprint: distance or count above sprint threshold; Acc: accelerations; Dec: decelerations; HI Acc/HI Dec: high-intensity accelerations/decelerations; TRIMPMOD: modified training impulse (HR-based); Edwards’ TL: heart-rate-zone weighted training load; RPEres TL: respiratory RPE-based training load; RPEmus TL: muscular RPE-based training load; HHb: deoxygenated hemoglobin; O2Hb: oxygenated hemoglobin; tHb: total hemoglobin; HRV: heart-rate variability; RMSSD: root mean square of successive differences; pNN50: percentage of NN intervals differing by >50 ms; SDNN: standard deviation of NN intervals; LF: low-frequency HRV power; HF: high-frequency HRV power; LF/HF: low- to high-frequency power ratio; lnRMSSD/lnSDNN/lnSD1/lnHF/lnLF: log-transformed HRV indices; Temp: tympanic temperature; GNG: go/no-go task; GNGt: go/no-go response time; GNGe: go/no-go errors; MSR: moderate-speed running; MV: mean velocity; MD-4: match day minus four.