Table 1. PCR primers for MMPs, TIMPs, and GAPDH
gene sense and antisense primers cDNA size (bp)
MMP-1 5′-CACAGCTTTCCTCCACTGCTGCTGC-3′
5′-GGCATGGTCCACATCTGCTCTTGGC-3′
396
MMP-2 5’-GACAAGAACCAGATCAATAC-3’
5’-GCCATGCTCCCAGCGGCCAAA-3’
181
MMP-13 5′-TGGTGGTGATGAAGATGAT TTGTCT-3′
5′-AGTTACATCGGACCAAACTTTGAAG-3′
366
TIMP-1 5′-CCTGGCTTCTGGCATCCTGTT-3′
5′-GGGACCTGTGGAAGTATCCG C-3′
280
TIMP-2 5′-CAGTGAGAAGGAAGTGGACTC-3′
5′-CATCTGGTACCTGTGGTTCAG-3′
300
TIMP-3 5’-ACGCCTTCTGCAACTCCGACA-3’
5’-CCTCTCAGCAGGTACTGGTAC-3’
221
GAPDH 5′-CCACCCATGGCAAATTCCA TGGCA-3′
5′-TCTAGACGGCAGGTCAGGTCCACC-3′
600