Table 1 Common Platelet-rich Therapy Preparation Systems.
System Volume
Of Blood
(mL)
Centrifuge
Time/Speed
Final PRP
Volume
(mL)
Final Platelet
Concentration
(compared with
average)
Activator Level of Growth
Factors (compared
with average)
Autologous Conditioned Plasma (Arthrex, Naples, FL) 9 5 min/1,500 rpm 3-5 2-3x None PDGF (25x)
EGF (5x)
VEGF (11x)
TGF-β1 (4x)
IGF-1 (1x)
Cascade (Musculoskeletal Tissue
Foundation, Edison, NJ)
9 or 18 First: 6 min/1,100g;
Second: 15 min/
1,450g
2 or 4 N/A Calcium
(forms a
suturable
clot for
intraoperative
use)
PDGF (N/A)
EGF (5-10x)
VEGF (5-10x)
TGF-β1 (5-10x)
IGF-1 (5-10x)
GPS III (Biomet, Warsaw, IN)§ 27 or 54 15 min/1,900g 3 or 6 4-8x Calcium
chloride/
thrombin
PDGF (N/A)
EGF (3.9x)
VEGF (6.2x)
TGF-β1 (3.6x)
IGF-1 (1x)
SmartPReP
(Harvest Technologies, Plymouth,
MA)
20 or 60 14 min/1,000g 3 or 7 4.4-7.6x Thrombin PDGF (4.4×)
EGF (4.4×)
VEGF (4.4×)
TGF-β1 (4.4×)
IGF-1 (N/A)
* Information obtained from product manufacturers (platelet and growth factor concentration obtained from unpublished company data for all products listed except for Biomet GPS III [Eppley et al.])
† Arthrex: https://www.arthrex.com/innovations/index.cfm?adid=28&CFID=2168033&CFTOKEN=63754575
‡ Musculoskeletal Tissue Foundation: http://platelettherapy.com/
§ Biomet: http://www.biomet.com/biologics/information/pdf/BBI0003.0.pdf
‖ Harvest Technologies: http://www.harvesttech.com/products/smartprepmain.html
EGF = epidermal growth factor, IGF-1 = insulin-like growth factor-1, N/A = not available, PDGF = platelet-derived growth factor
PRP = platelet-rich plasma
TGF-β1 = transforming growth factor-β1
VEGF = vascular endothelial growth factor