Supplementary Table S4. Myotonometry characteristics.
Period Sequence Pre Δ3′ Δ6′ Δ9′ Δ12′
Stiffness [N/m] (M ± SD; 95%CI)
P1 ICA→VPA 296.43 ± 20.30 (277.65; 315.21) -0.29 ± 9.11
(-8.71; 8.14)
-6.43 ± 8.10
(-13.92; 1.06)
-1.71 ± 6.34
(-7.58; 4.15)
-5.43 ± 4.31
(-9.42; -1.44)
VPA→ICA 314.00 ± 24.64 (291.21; 336.79) -2.14 ± 10.07
(-11.46; 7.17)
-3.57 ± 8.20
(-11.16; 4.01)
-4.14 ± 10.29
(-13.66; 5.37)
-10.00 ± 23.78
(-31.99; 11.99)
P2 ICA→VPA 284.14 ± 14.25 (270.96; 297.32) 2.14 ± 7.47
(-4.77; 9.05)
1.43 ± 10.39
(-8.18; 11.04)
-3.14 ± 9.86
(-12.26; 5.97)
2.86 ± 8.71
(-5.20; 10.91)
VPA→ICA 296.71 ± 28.48 (270.37; 323.06) 5.14 ± 17.95
(-11.46; 21.74)
3.43 ± 11.06
(-6.80; 13.66)
6.57 ± 14.11
(-6.47; 19.62)
1.43 ± 12.43
(-10.07; 12.93)
Decrement (M ± SD; 95%CI)
P1 ICA→VPA 1.41 ± 0.26
(1.17; 1.65)
-0.09 ± 0.05
(-0.14; -0.04)
-0.06 ± 0.12
(-0.18; 0.05)
-0.07 ± 0.10
(-0.16; 0.02)
-0.01 ± 0.07
(-0.07; 0.06)
VPA→ICA 1.40 ± 0.31
(1.12; 1.69)
0.00 ± 0.09
(-0.08; 0.09)
0.04 ± 0.15
(-0.10; 0.18)
0.01 ± 0.14
(-0.13; 0.14)
-0.05 ± 0.10
(-0.14; 0.04)
P2 ICA→VPA 1.67 ± 0.20
(1.48; 1.85)
-0.11 ± 0.16
(-0.26; 0.04)
-0.11 ± 0.15
(-0.25; 0.03)
-0.13 ± 0.18
(-0.30; 0.03)
-0.08 ± 0.14
(-0.21; 0.05)
VPA→ICA 1.71 ± 0.24
(1.48; 1.93)
-0.04 ± 0.12
(-0.15; 0.07)
-0.08 ± 0.14
(-0.21; 0.05)
-0.03 ± 0.13
(-0.16; 0.09)
0.03 ± 0.20
(-0.15; 0.22)
Freqeuncy [Hz] (M ± SD; 95%CI)
P1 ICA→VPA 15.74 ± 1.42
(14.43; 17.05)
0.07 ± 0.71
(-0.59; 0.73)
-0.07 ± 0.59
(-0.61; 0.47)
0.10 ± 0.60
(-0.46; 0.66)
-0.13 ± 0.52
(-0.61; 0.35)
VPA→ICA 16.76 ± 0.91
(15.91; 17.60)
0.06 ± 0.72
(-0.61; 0.72)
-0.01 ± 0.92
(-0.87; 0.84)
0.13 ± 0.71
(-0.53; 0.79)
-0.48 ± 1.29
(-1.68; 0.71)
P2 ICA→VPA 15.93 ± 0.85
(15.15; 16.71)
0.64 ± 0.81
(-0.10; 1.39)
0.54 ± 0.58
(0.01; 1.08)
0.43 ± 0.75
(-0.27; 1.13)
0.31 ± 0.46
(-0.11; 0.74)
VPA→ICA 16.79 ± 1.61
(15.30; 18.27)
0.26 ± 0.92
(-0.59; 1.11)
0.20 ± 0.94
(-0.67; 1.07)
0.33 ± 0.75
(-0.36; 1.02)
0.07 ± 0.78
(-0.65; 0.80)

Values are mean ± SD (95% CI), with CI in round brackets using a semicolon (e.g., (lower; upper). Pre: measurement after the standardized general warm-up in that period. Δ3′/Δ6′/Δ9′/Δ12′: change from Pre at 3/6/9/12 min (positive = increase; negative = decrease). Period: P1 and P2 in the crossover design. Sequence: order of conditions—ICA→VPA (P1: isometric squat ICA; P2: volleyball activation without ICA) and VPA→ICA (reverse). Conditions: ICA = isometric conditioning activity (3×5×3 s; total 45 s; 3-min interset rest); VPA— standardized volleyball pre-training activation condition.