The aging process promotes body fat storage and physical deterioration due to the progressive loss of muscle strength (Bouchard et al., 2009). The loss of muscle function accompanying aging was strongly associated with mobility and autonomy impairment, falls, and mortality in severe cases (Bouchard and Janssen, 2010). As the global aging population increases, the research community seeks effective interventions to address these concerning health issues. Concurrently, older adults are more likely to become overweight due to a slower metabolic rate (Al-Nimr, 2019). Overweight was a significant factor that triggers various metabolic diseases, such as hypertension and diabetes (Marques et al., 2018).

One of the most outstanding strategies to conserve muscle mass and strength among overweight older adults is to practice regular physical exercise (Saint-Maurice et al., 2019). For instance, resistance and aerobic training have been shown to enhance endurance, muscle strength (Li et al., 2021a), functional ability (Li et al., 2021b), as well as reduce obesity among older adults (Barbat-Artigas et al., 2014). Therefore, exercise is a promising non-pharmacological means of boosting physical fitness among overweight older adults.

Meanwhile, the citrulline (CIT) amino acid has been used to alleviate physical performance in older adults. Oral CIT supplementation has also been proven to boost skeletal muscle mass and muscle protein synthesis, fiber size, and strength (Osowska et al., 2006; Faure et al., 2012, 2013), besides reducing adipose tissue, as observed in old malnourished rats (Faure et al., 2013). In fact, a systematic review described that CIT supplementation could benefit the older adults with various comorbidities, such as malnourished, hypertensive, obese, and dynapenic-obese (Aubertin-Leheudre and Buckinx, 2020). Moreover, CIT supplementation promotes lean muscle formation (Bouillanne et al., 2019) and aerobic capacity (Ashley et al., 2018) and reduces fat mass (Figueroa et al., 2015) compared to placebo (PLA).

CIT is found abundantly in fruits, such as watermelon, and is synthesized from arginine and ornithine, with plasma glutamine and arginine as the main precursors (Marini, 2012). It contributes to arginine bioavailability and the subsequent nitric oxide (NO) synthesis due to the role of CIT as a precursor to arginine (Viribay et al., 2022). Nevertheless, CIT differs from arginine as it is directly transported to the kidneys without arginase enzyme catabolism (Breuillard et al., 2015). Additionally, CIT could limit arginase action and arginine to ornithine catabolism (Figueroa et al., 2017). Thus, dietary CIT is potentially superior to arginine supplementation in boosting arginine bioavailability and NO synthesis (Marini, 2012; Figueroa et al., 2017). CIT may be able to improve peripheral vascular relaxation/perfusion and subsequent muscle O₂ utilization by increasing the bioavailability of NO in active skeletal muscles, thereby enhancing exercise performance (Wang et al., 2001; Smith et al., 2002).

Interestingly, past literature has reported that combined exercise training and CIT supplementation may be beneficial for improving body composition and function. One study by Buckinx et al. (2020) compared the effect of intervention among overweight older adults. The intervention protocol was performed thrice weekly for 12 weeks. The experimental group was subjected to 85% maximum heart rate (HR_max) of High-intensity Interval Training (HIIT) with CIT supplementation (10 g/day tablets), while the control group underwent HIIT combined with PLA supplementation with the same dose as CIT supplementation. The results showed that those who exercised and consumed CIT in the experimental group exhibited improved fat and lean mass compared to those in the control group. However, Marcangeli et al. (2022) applied the same intervention plan and reported a contradicting finding. The study found that the fat and lean mass between the two groups were insignificantly different.

Despite the potential benefits of CIT supplementation and exercise on overweight older adults, no meta-analysis has been performed to examine this intriguing correlation. Therefore, this meta-analysis was conducted to explore the effects of combined exercise with CIT supplementation on body composition and lower limb function in older overweight adults.

The current study explored the impact of combined exercise with CIT supplementation on the body composition and lower limb function of overweight older adults. Based on the developed search strategy, seven studies with participants’ BMI greater than 25 kg/m² were selected for the meta-analysis. The findings showed that the combined exercise with CIT supplementation enhanced 6MWT and LLS among overweight older adults more than with PLA supplementation. However, body composition was unaffected by the intervention protocol.

The study findings also suggested that the CIT supplementation effectively improved the physical function of overweight older adults, consistent with earlier reports. For instance, a six-week exercise program with CIT supplementation (3g/day) (Caballero-García et al., 2021) (N = 44) reported significant improvements in the walking speed of older adults in the CIT group compared to those in the PLA group. The results may be attributed to the enhanced muscle strength post-intervention. Therefore, CIT supplementation was considered a viable alternative rehabilitation plan for overweight older adults to improve their physical function.

Muscle strength is an indicator of daily physical function among older adults. Reduced physical activity results in weakened lower limbs and causes anabolic resistance, which is critical for regaining muscle mass (Breen et al., 2013; Devries et al., 2015). Muscle strength also declines with age, particularly in the legs, impairing physical performance and increasing the mortality risk (Manini and Clark, 2012). Therefore, overweight older adults should maintain their leg muscle mass and strength (Janssen et al., 2000). This review noted a more significant improvement in muscle strength in the CIT group than in the PLA group, which agrees with previous reports (Buckinx et al., 2020; Kang et al., 2022).

Although the mechanism of increased muscle mass is currently unclear, the increase in exercise performance may be related to changes in oxygen (O₂) transport and more regular utilization of skeletal muscles. Another study reported the impact of one week oral CIT supplementation (6 g/day) on muscle O₂ delivery and utilization in healthy young adults to increase muscle oxygenation index and lower the pattern of muscle deoxyhemoglobin during high-intensity cycling (Bailey et al., 2015). It was postulated that improving O₂ availability and distribution within the muscle microvasculature enhanced their performance during exercise.

Besides, the effect of CIT supplementation (2.4 g/day) for a week on healthy trained men was previously assessed (Suzuki et al., 2016). Following the CIT supplementation, they were able to complete 4 km of cycling for a much shorter time with increased power output, although no significant difference in O₂ consumption between the PLA and CIT groups was detected. Likewise, male athletes supplemented with CIT (3 g/day) for a week recorded an improved pedaling speed and average power output (Terasawa and Nakada, 2019).

The recent study also revealed that CIT supplementation resulted in exercise performance and anti-fatigue during high-intensity exercise (Terasawa and Nakada, 2019). This improvement could be attributed to enhanced peripheral vasodilation and perfusion. The benefits of CIT on exercise performance may be attributed to improving peripheral vascular relaxation/perfusion and subsequent muscle O₂ utilization by increasing the bioavailability of NO in active skeletal muscles, as studies have shown that CIT may be an important source of NO and may affect muscle function (Wang et al., 2001; Smith et al., 2002). CIT supplementation could also prevent the elevation of exercise-induced blood ammonia in mice, resulting in prolonged exhaustion (Takeda et al., 2011).

Intense exercise and fatigue lead to ammonia accumulation in the muscle, which is associated with various functional and metabolic neurological disturbances. Furthermore, ammonia accumulation was reported to inhibit mitochondrial oxidation of pyruvate to acetyl-CoA and reduced ATP output via the Krebs cycle, leading to muscle fatigue (Mutch and Banister, 1983; Takeda et al., 2011). Since CIT is a component of the urea cycle, CIT supplementation may promote ammonia elimination via the urea cycle, but the mechanism has yet to be elucidated. Hence, an in-depth mechanism of ammonia elimination through CIT supplementation should be examined.

It is worth highlighting several limitations in this review study. First, the sample size only encompassed seven studies, which was considered fairly small. In addition, the review solely considered the combined effects of exercise with CIT supplementation. Thus, the impact of CIT supplementation alone on the measured outcomes was unknown. Therefore, the intervention results were interpreted exclusively based on the influence of exercise. It is recommended that future research should include more high-quality RCT studies with varying populations to improve the accuracy and impact of the study outcome.

The meta-analysis in this study demonstrated that combined exercise with CIT supplementation could be an effective non-pharmaceutical intervention to improve the physical function of overweight older adults by increasing their muscle strength.