Journal of Sports Science and Medicine
Journal of Sports Science and Medicine
ISSN: 1303 - 2968   
Ios-APP Journal of Sports Science and Medicine
Androit-APP Journal of Sports Science and Medicine
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©Journal of Sports Science and Medicine ( 2015 ) 14 , 203 - 214

Research article
Reliability and Accuracy of Six Hand-Held Blood Lactate Analysers
Jacinta M. Bonaventura1, Ken Sharpe2, Emma Knight3, Kate L. Fuller1, Rebecca K. Tanner1, Christopher J. Gore1,4, 
Author Information
1 Physiology, Australian Institute of Sport, Canberra, Australian Capital Territory, Australia
2 Department of Mathematics and Statistics, The University of Melbourne, Parkville, Victoria, Australia
3 Performance Research, Australian Institute of Sport, Canberra, Australian Capital Territory, Australia
4 Exercise Physiology Laboratory, Flinders University, Bedford Park, South Australia, Australia

Christopher J. Gore
✉ Australian Institute of Sport, PO Box 176, Belconnen ACT 2617, Australia
Email: chris.gore@ausport.gov.au
Publish Date
Received: 14-08-2014
Accepted: 18-11-2014
Published (online): 01-03-2015
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ABSTRACT

The reliability and accuracy of five portable blood lactate (BLa) analysers (Lactate Pro, Lactate Pro2, Lactate Scout+, Xpress™, and Edge) and one handheld point-of-care analyser (i-STAT) were compared to a criterion (Radiometer ABL90). Two devices of each brand of analyser were assessed using 22 x 6 mL blood samples taken from five subjects at rest and during exercise who generated lactate ranging ~1-23 mM. Each sample was measured simultaneously ~6 times on each device. Reliability was assessed as the within-sample standard deviation (wsSD) of the six replicates; accuracy as the bias compared with the ABL90; and overall error (the root mean squared error (√MSE)) was calculated as the square root of (wsSD2 and bias2). The √MSE indicated that both the Edge and Xpress had low total error (~0-2 mM) for lactate concentrations <15 mM, whereas the Edge and Lactate Pro2 were the better of the portable analysers for concentrations >15 mM. In all cases, bias (negative) was the major contribution to the √MSE. In conclusion, in a clinical setting where BLa is generally <15 mM the Edge and Xpress devices are relevant, but for athlete testing where peak BLa is important for training prescription the Edge and Lactate Pro2 are preferred.

Key words: Bias, precision, root mean squared error, analytical performance


           Key Points
  • The reliability of five common portable blood lactate analysers were generally <0.5 mM for concentrations in the range of ~1.0-10 mM.
  • For all five portable analysers, the analytical error within a brand was much smaller than the biological variation in blood lactate (BLa).
  • Compared with a criterion blood lactate analyser, there was a tendency for all portable analysers to under-read (i.e. a negative bias), which was particularly evident at the highest concentrations (BLa ~15-23 mM).
  • The practical application of these negative biases would overestimate the ability of the athlete and prescribe a training intensity that would be too high.
 
 
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