Journal of Sports Science and Medicine
Journal of Sports Science and Medicine
ISSN: 1303 - 2968   
Ios-APP Journal of Sports Science and Medicine
Androit-APP Journal of Sports Science and Medicine
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©Journal of Sports Science and Medicine ( 2015 ) 14 , 297 - 303

Research article
Microcurrent Electrical Neuromuscular Stimulation Facilitates Regeneration of Injured Skeletal Muscle in Mice
Hiroto Fujiya1,5, , Yuji Ogura2, Yoshitaka Ohno3, Ayumi Goto4, Ayane Nakamura4, Kazuya Ohashi4, Daiki Uematsu3, Haruhito Aoki5, Haruki Musha1, Katsumasa Goto3,4
Author Information
1 Department of Sports Medicine, St. Marianna University School of Medicine, Kawasaki, Kanagawa, Japan
2 Department of Physiology, St. Marianna University School of Medicine, Kawasaki, Kanagawa, Japan
3 Laboratory of Physiology, School of Health Sciences, Toyohashi SOZO University, Toyohashi, Aichi, Japan
4 Department of Physiology, Graduate School of Health Sciences, Toyohashi SOZO University, Toyohashi, Aichi, Japan
5 St.Marianna University School of Medicine, FIFA Medical Centre of Excellence at Kawasaki, Kawasaki, Kanagawa, Japan

Hiroto Fujiya
‚úČ Department of Sports Medicine, St. Marianna University School of Medicine, Kawasaki, 2-16-1 Sugao, Miyamae, Kawasaki, Kanagawa, 216-8511, Japan
Email: fujiya-1487@marianna-u.ac.jp
Publish Date
Received: 26-09-2014
Accepted: 27-01-2015
Published (online): 01-06-2015
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ABSTRACT

Conservative therapies, mainly resting care for the damaged muscle, are generally used as a treatment for skeletal muscle injuries (such as muscle fragmentation). Several past studies reported that microcurrent electrical neuromuscular stimulation (MENS) facilitates a repair of injured soft tissues and shortens the recovery period. However, the effects of MENS on the regeneration in injured skeletal muscle are still unclear. The purpose of this study was to investigate the effect of MENS on the regenerative process of injured skeletal muscle and to elucidate whether satellite cells in injured skeletal muscle are activated by MENS by using animal models. Male C57BL/6J mice, aged 7 weeks old, were used (n = 30). Mice were randomly divided into two groups: (1) cardiotoxin (CTX)-injected (CX, n = 15) and (2) CTX-injected with MENS treatment (MX, n=15) groups. CTX was injected into tibialis anterior muscle (TA) of mice in CX and MX groups to initiate the necrosis-regeneration cycle of the muscle. TA was dissected 1, 2, and 3 weeks after the injection. Muscle weight, muscle protein content, the mean cross-sectional areas of muscle fibers, the relative percentage of fibers having central nuclei, and the number of muscle satellite cells were evaluated. MENS facilitated the recovery of the muscle dry weight and protein content relative to body weight, and the mean cross-sectional areas of muscle fibers in CTX-induced injured TA muscle. The number of Pax7-positive muscle satellite cells was increased by MENS during the regenerating period. Decrease in the percentages of fibers with central nuclei after CTX-injection was facilitated by MENS. MENS may facilitate the regeneration of injured skeletal muscles by activating the regenerative potential of skeletal muscles.

Key words: Muscle injury, regenerative potential, muscle satellite cell, central nuclei, physiotherapy, sports injury


           Key Points
  • Microcurrent electrical neuromuscular stimulation (MENS) facilitated the recovery of the relative muscle dry weight, the relative muscle protein content, and the mean cross-sectional areas of muscle fibers of injured TA muscle in mice.
  • The number of satellite cells was increased by MENS during the regenerating phase of injured skeletal muscle.
  • Decrease in the percentages of fibers with central nuclei was facilitated by MENS.
  • MENS may facilitate the regeneration of injured skeletal muscles.
 
 
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