Journal of Sports Science and Medicine
Journal of Sports Science and Medicine
ISSN: 1303 - 2968   
Ios-APP Journal of Sports Science and Medicine
Androit-APP Journal of Sports Science and Medicine
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©Journal of Sports Science and Medicine ( 2019 ) 18 , 146 - 154

Research article
Alteration of Autophagy Gene Expression by Different Intensity of Exercise in Gastrocnemius and Soleus Muscles of Wistar Rats
Vita Murniati Tarawan1, Julia Windi Gunadi2, , Setiawan1, Ronny Lesmana1,3, Hanna Goenawan1,3, Debby Eka Meilina4, Julidea Anggiriani Sipayung4, Teresa Liliana Wargasetia5, Wahyu Widowati5, Yenni Limyati6, Unang Supratman3,7
Author Information
1 Department of Biomedical Science, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia
2 Department of Physiology, Faculty of Medicine, Maranatha Christian University, Bandung, Indonesia
3 Division of Biological Activity, Central Laboratory, Universitas Padjadjaran, Bandung, Indonesia
4 Faculty of Medicine, Maranatha Christian University, Bandung, Indonesia
5 Department of Biology, Faculty of Medicine, Maranatha Christian University, Bandung, Indonesia
6 Physical Medicine and Rehabilitation Department, Immanuel Hospital, Bandung, Indonesia
7 Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Bandung, Indonesia

Julia Windi Gunadi
✉ Department of Physiology, Jalan drg.Suria Sumantri no. 65, Bandung, West Java, Indonesia
Email: juliawindig@gmail.com
Publish Date
Received: 02-12-2018
Accepted: 16-01-2019
Published (online): 11-02-2019
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ABSTRACT

Exercise-induced skeletal muscle adaptation requires degradation of cellular components carried out by autophagy. However, the alteration of autophagy by different intensity of exercise in skeletal muscle is still unknown. In the present study, we investigate whether low, moderate, and high-intensity exercises have different impacts on autophagy gene expression in gastrocnemius and soleus muscles of wistar rats. This work is limited because only rats are used, and does not cover human tissues. Twenty male wistar rats were assigned to four groups: sedentary control, low-intensity (LI, 10 m/minute), moderate-intensity (MI, 20 m/minute), and high-intensity (HI, 30 m/minute) exercises. Training was conducted 30 minutes/day with a 5 times/week interval for 8 weeks. RNA and protein were extracted from gastrocnemius and soleus muscles then stored in -80°C. Specific primers and antibodies for autophagy genes and protein levels were utilized for semi-quantitative PCR and Western Blot. Exercises decrease expression of autophagy gene LC3 (LI and MI 0.7 fold, HI 0.8 fold, p < 0.05) in gastrocnemius muscles and soleus muscle (LI, MI, and HI 0.8 fold, p < 0.05) compared to control. On the other side, we observed p62 gene expression decreased in gastrocnemius (0.8 fold, p < 0.05) and soleus (0.9 fold, p < 0.05) muscles with MI, but increased in soleus (1.1 fold, p < 0.05) muscles with HI. This result is consistent with the change of protein level, suggesting that autophagy might be modulated by different type of exercise. This study suggests that intensity of exercise and different type of muscle fibers effect autophagy gene expression in skeletal muscle of wistar rats. MI exercise increases autophagy gene expression in gastrocnemius and soleus muscles, but HI exercise decreases autophagy gene expression in soleus muscles of wistar rats. Soleus muscles are more responsive to exercise compared to gastrocnemius muscles.

Key words: Autophagy, exercise, gastrocnemius, soleus, LC3, p62


           Key Points
  • Autophagy in skeletal muscle is altered by different intensity of exercise
  • Moderate intensity of exercise may increase autophagy in gastrocnemius and soleus muscles of wistar rats.
  • High intensity of exercise may decrease autophagy in soleus muscles of wistar rats.
  • Different types of muscle also affected autophagy gene expression in skeletal muscles of wistar rats.
 
 
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