Journal of Sports Science and Medicine
Journal of Sports Science and Medicine
ISSN: 1303 - 2968   
Ios-APP Journal of Sports Science and Medicine
Androit-APP Journal of Sports Science and Medicine
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©Journal of Sports Science and Medicine (2006) 05, 682 - 691

Research article
Influence Of Chronic Exercise On Red Cell Antioxidant Defense, Plasma Malondialdehyde And Total Antioxidant Capacity In Hypercholesterolemic Rabbits
Mohsen Alipour1, Mustafa Mohammadi2, , Nosratollah Zarghami3, Nasser Ahmadiasl2
Author Information
1 Department of Physiology, Zanjan University of Medical Sciences, Zanjan, Iran
2 Department of Physiology,
3 Depatment of Clinical Biochemistry and RIA, Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

Mustafa Mohammadi
‚úČ Department of Physiology, Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Email: m.mohammadin@yahoo.com
Publish Date
Received: 11-05-2005
Accepted: 26-10-2006
Published (online): 15-12-2006
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ABSTRACT

Despite the knowledge on the antiatherogenic effects of exercise, the mechanism by which exercise reduces atherogenic risk remains unknown. In this study, we investigated the hypothesis that chronic exercise-induced oxidative stress may increase plasma total antioxidant capacity and antioxidant defense in the red cells. For 8 weeks, 60 male Dutch rabbits were fed rabbit chow with or without the addition of 2% cholesterol. The animals were further divided into rest and exercise groups (n = 15 for each group). Animals in exercise groups ran on a rodent treadmill at 15 m/min for 10 to 60 minutes gradually for 5 days per week for a total of 8 weeks. At the end of experiments, blood samples were collected and glutathione peroxidase (GPX), superoxide dismutase (SOD), and catalase (CAT) activities were determined in red blood cells. Total antioxidant capacity (TAC), malondialdehyde (MDA) and total thiol (T-SH) levels were measured in plasma. Thoracic aorta and carotid arteries were isolated for histological examination to evaluate atherosclerosis. Eight weeks of chronic exercise reduced atherogenic diet-induced atherosclerotic lesions in all the arteries studied, along with positive changes in cholesterol profile, especially increase of serum HDL-C level. Plasma MDA, TAC and T-SH concentrations were enhanced by exercise in both control and hypercholesterolemic diet groups. Erythrocyte catalase activity was significantly increased by chronic exercise (p < 0.05), whereas total SOD activity rose with exercise only in the control group. Surprisingly, GPX activity was significantly reduced (P < 0.05) in response to exercise in the control group and also in the high cholesterol diet group. Exercise is a useful tool for the prevention and regression of atherosclerosis which is evident by our findings of the enhancement of plasma TAC and positive change in serum cholesterol profile. However, the effect of exercise on red cell antioxidant activities is limited in the hypercholesterolemic animals compared to control animals, possibly in part because of alterations in the ability to adapt to exercise-induced oxidative stress in high cholesterol diet.

Key words: Chronic exercise, antioxidant, malondialdehyde, thiol, atherosclerosis


           Key Points
  • Plasma MDA, TAC and T-SH concentrations were enhanced by exercise in both control and high cholesterol diet groups.
  • GPX activity was significantly reduced in response to exercise in the control group and also in the high cholesterol diet group.
  • Eight weeks of chronic exercise reduced atherogenic diet-induced atherosclerotic lesions in all the arteries studied.
 
 
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