We investigated whether single or combined methods of pre-cooling could affect high-intensity exercise performance in a hot environment. Seven male athletes were subjected to four experimental conditions for 30 min in a randomised order. The four experimental conditions were: 1) wearing a vest cooled to a temperature of 4 ™ƒ (Vest), 2) consuming a beverage cooled to a temperature of 4 ™ƒ (Beverage), 3) simultaneous usage of vest and consumption of beverage (Mix), and 4) the control trial without pre-cooling (CON). Following those experimental conditions, they exercised at a speed of 80% VO₂max until exhaustion in the heat (38.1 ± 0.6 ™ƒ, 55.3 ± 0.3% RH). Heart rate (HR), rectal temperature (T_core), skin temperature (T_skin), sweat loss (SL), urine specific gravity (USG), levels of sodium (Na⁺) and potassium (K⁺), rating of perceived exertion (RPE), thermal sensation (TS), and levels of blood lactic acid ([Bla]) were monitored. Performance was improved using the mixed pre-cooling strategy (648.43 ± 77.53 s, p = 0.016) compared to CON (509.14 ± 54.57 s). T_core after pre-cooling was not different (Mix: 37.01 ± 0.27 ™ƒ, Vest: 37.19 ± 0.33 ™ƒ, Beverage: 37.03 ± 0.35 ™ƒ) in all cooling conditions compared to those of CON (37.31 ±0.29 ™ƒ). A similar T_core values was achieved at exhaustion in all trials (from 38.10 ™ƒ to 39.00 ™ƒ). No difference in the level of USG was observed between the conditions. Our findings suggest that pre-cooling with a combination of cold vest usage and cold fluid intake can improve performance in the heat.

• Precooling strategies with 30 min independent or simultaneous precooling maneuvers could improve running performance of 80% VO₂max in hot and humid climatic conditions compared with the control condition.

• Wearing a 4™ƒ vest might augment the capacity of heat dissipation by decreasing T_skin.

• Ingesting a 4™ƒ beverage might increase the capacity of heat storage and maintain homeostasis by reducing T_core, HR and [Bla].

• The mix method-precooling maneuver seems to be more effective than others by maintaining core-to-skin thermal gradient and fortifying heat storage to relieve thermophysiological strain.