Journal of Sports Science and Medicine
Journal of Sports Science and Medicine
ISSN: 1303 - 2968   
Ios-APP Journal of Sports Science and Medicine
Androit-APP Journal of Sports Science and Medicine
©Journal of Sports Science and Medicine (2024) 23, 317 - 325   DOI:

Research article
Effects of Omega-3 Supplementation on the Delayed Onset Muscle Soreness after Cycling High Intensity Interval Training in Overweight or Obese Males
Niromlee Makaje1, Ratree Ruangthai1, Sudathip Sae-tan2, 
Author Information
1 Department of Sports Science and Health, Faculty of Sports Science, Kasetsart University, Bangkok, Thailand
2 Department of Food Science and Technology, Faculty of Agro-Industry, Kasetsart University, Bangkok, Thailand

Sudathip Sae-tan
‚úČ 50 Ngamwongwan Road Ladyao Chatuchak Bangkok, 10900 Thailand
Publish Date
Received: 16-01-2024
Accepted: 05-04-2024
Published (online): 01-06-2024

People with overweight or obesity preferred high-intensity interval training (HIIT) due to the time-efficiency and pleasure. However, HIIT leads to delayed onset muscle soreness (DOMS). The present study aimed to investigate the effects of omega-3 supplementation on DOMS, muscle damage, and acute inflammatory markers induced by cycling HIIT in untrained males with overweight or obesity. A randomized, double-blinded study was used in the present study. Twenty-four males with a sedentary lifestyle were randomly assigned to either receive omega-3 (O3) (4 g fish oil) or placebo (Con). Subjects consumed the capsules for 4 weeks and performed cycling HIIT at the 4th week. After 4 weeks-intervention, the omega-3 index of O3 group increased by 52.51% compared to the baseline. All subjects performed HIIT at 4th week. The plasma creatine kinase (CK) level of Con group increased throughout 48h after HIIT. While the CK level of O3 group increased only immediately and 24h after HIIT and decreased at 48h after HIIT. The white blood cell count (WBC) of Con group increased immediately after the HIIT, while O3 group did not show such increase. There was no change of CRP in both groups. O3 group had a higher reduction of calf pain score compared to Con group. O3 group also showed a recovery of leg strength faster than Con group. Omega-3 supplementation for 4 weeks lower increased CK level, reduced calf pain score, and recovery leg strength, DOMS markers after cycling HIIT.

Key words: Delayed onset muscle soreness, fish oil, high intensity interval training, muscle damage, obesity

           Key Points
  • Omega-3 supplementation 4g/d for 4 weeks increased omega-3 index in males with overweight or obesity.
  • Omega-3 supplementation reduced creatine kinase and pain scores, while increased muscle strength after the first cycling HIIT more rapidly than placebo.
  • Omega-3 supplementation could reduce DOMS and inflammatory markers induced by cycling HIIT.
  • The present results can be applied to people with overweight or obesity who desired to exercise for weight management but encounter DOMS after exercise.
Home Issues About Authors
Contact Current Editorial board Authors instructions
Email alerts In Press Mission For Reviewers
Archive Scope
Supplements Statistics
Most Read Articles
  Most Cited Articles
JSSM | Copyright 2001-2024 | All rights reserved. | LEGAL NOTICES | Publisher

It is forbidden the total or partial reproduction of this web site and the published materials, the treatment of its database, any kind of transition and for any means, either electronic, mechanic or other methods, without the previous written permission of the JSSM.

This work is licensed under a Creative Commons License Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.